Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2020 Jun;44(6):e13218.
doi: 10.1111/jfbc.13218. Epub 2020 Mar 31.

Barley α-amylase/subtilisin inhibitor shows inhibitory activity against endogenous xylanase isozyme I of malted barley: A novel protein function

Junyong Sun  1   2   3   4 , Feng Xu  5 , Jian Lu  1   2   3   4
Affiliations

Barley α-amylase/subtilisin inhibitor shows inhibitory activity against endogenous xylanase isozyme I of malted barley: A novel protein function

Junyong Sun et al. J Food Biochem. 2020 Jun.

Abstract

Mashing process had little influence on the arabinoxylan content in the finished wort. In this paper, a protein with inhibitory activity against the endogenous xylanase isozyme I (X-I) of malted barley was extracted and purified using a combination of ion-exchange and size-exclusion chromatography. The protein was identified as barley α-amylase/subtilisin inhibitor (BASI). According to the amino acid sequence analysis, BASI was completely different from the previous reported xylanase inhibitors. BASI showed dosage-dependent inhibitory activity. BASI exhibited a maximum inhibitory activity at 50°C and pH 6.0. BASI inhibited X-I as a competitive manner. PRACTICAL APPLICATIONS: A protein with inhibitory activity against the major endogenous xylanase isozyme I (X-I) of malted barley was extracted, purified, and characterized, which was identified as barley α-amylase/subtilisin inhibitor (BASI). The results help brewers to achieve a better understanding of the mechanism of arabinoxylan degradation during mashing. BASI can be used as an indicator to screen microbial xylanases. The microbial xylanases insensitive to BASI would have obvious advantages in the degradation of arabinoxylan polymers and filterability improvement during mashing.

Keywords: arabinoxylan polymer; barley α-amylase/subtilisin inhibitor; endogenous xylanase; malted barley; xylanase inhibitor.

PubMed Disclaimer

References

REFERENCES

    1. Adav, S. S., Ravindran, A., & Sze, S. K. (2015). Quantitative proteomic study of Aspergillus fumigatus secretome revealed deamidation of secretory enzymes. Journal of Proteomics, 119, 154-168. https://doi.org/10.1016/j.jprot.2015.02.007
    1. Bradford, M. M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72, 248-254. https://doi.org/10.1016/0003-2697(76)90527-3
    1. Campenhout, S. V., & Volckaert, G. (2005). Differential expression of endo-β-1,4-xylanase isoenzymes X-I and X-II at various stages throughout barley development. Plant Science, 169(3), 512-522. https://doi.org/10.1016/j.plantsci.2005.05.003
    1. Daneri-Castro, S. N., Chandrasekar, B., Grosse-Holz, F. M., van der Hoorn, R. A., & Roberts, T. H. (2016). Activity-based protein profiling of hydrolytic enzymes induced by gibberellic acid in isolated aleurone layers of malting barley. FEBS Letters, 590(17), 2956-2962. https://doi.org/10.1002/1873-3468.12320
    1. Debyser, W. (1999). Arabinoxylan solubilisation during the production of Belgian white beer and a novel class of wheat proteins that inhibit endoxylanases (Doctoral thesis). University of Leuven, Leuven, Belgium.

Publication types

LinkOut - more resources