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Comment
. 2020 Jun;35(3):344-347.
doi: 10.1007/s12250-020-00218-1. Epub 2020 Apr 1.

Development of a Novel Reverse Transcription Loop-Mediated Isothermal Amplification Method for Rapid Detection of SARS-CoV-2

Renfei Lu  1 , Xiuming Wu  2 , Zhenzhou Wan  3 , Yingxue Li  2 , Lulu Zuo  2 , Jianru Qin  2   4 , Xia Jin  5 , Chiyu Zhang  6
Affiliations
Comment

Development of a Novel Reverse Transcription Loop-Mediated Isothermal Amplification Method for Rapid Detection of SARS-CoV-2

Renfei Lu et al. Virol Sin. 2020 Jun.

Erratum in

No abstract available

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Conflict of interest statement

The authors declare that they have no conflict of interest.

Figures

Fig. 1
Fig. 1
A Location of the primers in SARS-CoV-2 genome. B Sequence comparison among seven human coronaviruses (SARS-CoV2, SARS-CoV, MERS-CoV, OC43, HKU1, NL63 and 229E). C Cross-reactivity test of the novel SARS-CoV-2 RT-LAMP assay to other common respiratory viruses. Tested common respiratory viruses include HCoV-HKU-1, HCoV-NL63, HCoV-OC43, HCoV-229E, influenza A, B, and C viruses, parainfluenza viruses type 1–3, enterovirus, respiratory syncytial virus A and B groups, human rhinovirus, human metapneumovirus, adenovirus and bocavirus. RNA from a COVID-19 patient was used as positive control (PC). NTC, non-template control. D Sensitivity test of the novel SARS-CoV-2 RT-LAMP assay. Positive amplification was defined only when all three replicates are successfully amplified. NTC: non-template control. E Visual detection of SARS-CoV-2 by the colorimetric RT-LAMP assay. E-a RNA standards; E-b Clinical samples. NTC: non-template control.
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