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. 2020;29(5):492-498.
doi: 10.1159/000506878. Epub 2020 Apr 2.

Bleeding Index and Monocyte Chemoattractant Protein 1 as Gingival Inflammation Parameters after Chemical-Mechanical Retraction Procedure

Marko Igic  1 , Milena Kostic  2 , Jelena Basic  3 , Nebojsa Krunic  2 , Ana Pejcic  4 , Nikola Gligorijevic  2 , Aleksandra Milic Lemic  5
Affiliations

Bleeding Index and Monocyte Chemoattractant Protein 1 as Gingival Inflammation Parameters after Chemical-Mechanical Retraction Procedure

Marko Igic et al. Med Princ Pract. 2020.

Abstract

Objective: A widely used chemical-mechanical method of gingival retraction can cause gingival tissue damage. The aim of this study was to test the influence of the chemical-mechanical gingival retraction procedures on the gingival bleeding index (GBI) and the salivary concentration of monocyte chemoattractant protein 1 (MCP-1) as an indicator of inflammatory changes in the gingiva.

Materials and methods: The effects of 2 different retraction agents (aluminum chloride and ferric sulfate) were compared, particularly their tissue damaging effect during tooth preparation. Therefore, GBI values and the salivary concentration of MCP-1 were assessed during the chemical-mechanical method of gingival retraction in a homogenous group of respondents. The subjects (n = 60) were divided into 2 experimental groups (G1 and G2) regarding the need for tooth preparing and making artificial crowns. Each group was further divided into 2 subgroups (R1 and R2) according to the type of the gingival retraction agent used (aluminum chloride and ferric sulfate).

Results: Compared to the values at the study start, a statistically significant increase in GBI and salivary MCP-1 (p < 0.001) 1 day after gingival retraction agent application was observed in both experimental groups. After 72 h, the values were lower than in the second observation period but still statistically significantly higher compared to the study start (p < 0.001), which indicated the reversibility of the tissue changes.

Conclusion: Higher values of the investigated parameters were observed in the group of subjects with prepared teeth, and clinical changes were more pronounced after the use of ferric sulfate.

Keywords: Astringent; Gingival bleeding index; Inflammation; MCP-1; Monocyte chemoattractant protein; Retraction.

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Conflict of interest statement

The authors have no conflicts of interest to declare.

Figures

Fig. 1
Fig. 1
GBI values during the study period in groups G1 (a) and G2 (b) with regard to the gingival retraction agent used.
Fig. 2
Fig. 2
MCP-1 concentrations (pg/mL) during the study period in groups G1 (a) and G2 (b) with regard to the gingival retraction agent used.
See this image and copyright information in PMC

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References

    1. Tabassum S, Adnan S, Khan FR. Gingival retraction methods: A systematic review. J Prosthodont. 2017 Dec;26((8)):637–43. - PubMed
    1. Fazekas A, Csempesz F, Csabai Z, Vág J. Effects of pre-soaked retraction cords on the microcirculation of the human gingival margin. Oper Dent. 2002 Jul-Aug;27((4)):343–8. - PubMed
    1. Kostić I, Mihailović D, Najman S, Stojanović S, Kostić M. The rabbit gingival tissue response to retraction liquids and tetrahydrozoline. Vojnosanit Pregl. 2014 Jan;71((1)):46–51. - PubMed
    1. Nowakowska D, Saczko J, Kulbacka J, Choromanska A, Raszewski Z. Cytotoxic potential of vasoconstrictor experimental gingival retraction agents: in vitro study on primary human gingival fibroblasts. Folia Biol (Praha) 2012;58((1)):37–43. - PubMed
    1. Lodetti G, D'Abrosca F, Fontana P, Pavoni E, Gigola P. [Set up of in vitro methods able to detect the safety of astringent liquids] Minerva Stomatol. 2004 Jun;53((6)):361–7. - PubMed