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. 2020 Aug;77(8):1864-1870.
doi: 10.1007/s00284-020-01972-x. Epub 2020 Apr 3.

Complete Genome Sequence of Pantoea ananatis Strain NN08200, an Endophytic Bacterium Isolated from Sugarcane

Quan Zeng #  1 , GuoYing Shi #  1 , ZeMei Nong  1 , XueLian Ye  1 , ChunJin Hu  2
Affiliations

Complete Genome Sequence of Pantoea ananatis Strain NN08200, an Endophytic Bacterium Isolated from Sugarcane

Quan Zeng et al. Curr Microbiol. 2020 Aug.

Abstract

Stain NN08200 was isolated from the surface-sterilized stem of sugarcane grown in Guangxi province of China. The stain was Gram-negative, facultative anaerobic, non-spore-forming bacteria. The complete genome SNP-based phylogenetic analysis indicate that NN08200 is a member of the genus Pantoea ananatis. Here, we summarize the features of strain NN08200 and describe its complete genome. The genome contains a chromosome and two plasmids, in total 5,176,640 nucleotides with 54.76% GC content. The chromosome genome contains 4598 protein-coding genes, and 135 ncRNA genes, including 22 rRNA genes, 78 tRNA genes and 35 sRNA genes, the plasmid 1 contains 149 protein-coding genes and the plasmid 2 contains 308 protein-coding genes. We identified 130 tandem repeats, 101 transposon genes, and 16 predicted genomic islands on the chromosome. We found an indole pyruvate decarboxylase encoding gene which involved in the biosynthesis of the plant hormone indole-3-acetic acid, it may explain the reason why NN08200 stain have growth-promoting effects on sugarcane. Considering the pathogenic potential and its versatility of the species of the genus Pantoea, the genome information of the strain NN08200 give us a chance to determine the genetic background of interactions between endophytic enterobacteria and plants.

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Figures

Fig. 1
Fig. 1
Transmission electron microphotograph of the Pantoea ananatis strain NN08200
Fig. 2
Fig. 2
Phylogenetic tree based on the genome sequences showing the phylogenetic position of strain NN08200 and other strains belonging to the genus Pantoea. A PHYML method was beedn used to build the phylogenetic tree based on SNP of complete genomes for strain belonging to the genus Pantoea constructed by TreeBeST
Fig. 3
Fig. 3
Graphical circular map of the chromosome and plasmids of Pantoea ananatis NN08200 by Circos. From outside to the center: Coding genes on forward and reverse strands, the results of gene function annotation (including genes, COG, KEGG, GO), ncRNAs
See this image and copyright information in PMC

References

    1. Feng YJ, Shen DL, Dong XZ, et al. In vitro symplasmata formation in the rice diazotrophic endophyte Pantoea agglomerans YS19. Plant Soil. 2003;255(2):435–444. doi: 10.1023/A:1026079203474. - DOI
    1. Brady C, Cleenwerck I, Venter S, et al. Phylogeny and identiication of Pantoea species associated with plants, humans and the natural environment based on multilocus sequence analysis (MLSA) Syst Appl Microbiol. 2008;31:447–460. doi: 10.1016/j.syapm.2008.09.004. - DOI - PubMed
    1. Choi Q, Kim H, Lee Y, et al. First report of sheathrot of rice caused by Pantoea ananatis in Korea. Plant Pathol J. 2012;28(3):331. doi: 10.5423/PPJ.DR.08.2011.0150. - DOI
    1. Zhang J, Zhang CW, Yang J, et al. Insights into endophytic bacterial community structures of seeds among various Oryza sativa L. rice genotypes. J Plant Growth Regul. 2019;38(1):93–102. doi: 10.1007/s00344-018-9812-0. - DOI
    1. Volksch B, Thon S, Jacobsen I, et al. Polyphasic study of plant and clinic-associated Pantoea agglomerans strains reveals indistinguishable virulence potential. Infect Genet Evol. 2009;9:1381–1391. doi: 10.1016/j.meegid.2009.09.016. - DOI - PubMed

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