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Comment
. 2020;151(2 & 3):251-254.
doi: 10.4103/ijmr.IJMR_671_20.

Development of in vitro transcribed RNA as positive control for laboratory diagnosis of SARS-CoV-2 in India

Manohar Lal Choudhary  1 , Veena Vipat  1 , Sheetal Jadhav  1 , Atanu Basu  2 , Sarah Cherian  3 , Priya Abraham  4 , Varsha A Potdar  1
Affiliations
Comment

Development of in vitro transcribed RNA as positive control for laboratory diagnosis of SARS-CoV-2 in India

Manohar Lal Choudhary et al. Indian J Med Res. 2020.
No abstract available

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Conflict of interest statement

None

Figures

Fig. 1
Fig. 1
Positive DNA amplification of envelope (E), RNA-dependent RNA polymerase (RdRp), nucleocapsid (N) and open reading frame (ORF) 1b-nsp14 for in vitro transcribed preparation. Lane 1: E gene (550 bp), lane 2: RdRp gene (344 bp), lane 3: N gene (1254 bp) positive, lane 4: ORF (388 bp), lane 5: 100 bp DNA ladder.
Fig. 2
Fig. 2
(A-E) Multicomponent amplification graph for 10-fold dilutions of in vitro transcribed RNA: (A) E gene, (B) RdRp gene probe 1, (C) RdRp gene probe 2, (D) ORF gene, (E) N gene. The X axis represents number of cycles and Y axis represents amount of fluorescence.
See this image and copyright information in PMC

Comment on

  • Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR.
    Corman VM, Landt O, Kaiser M, Molenkamp R, Meijer A, Chu DK, Bleicker T, Brünink S, Schneider J, Schmidt ML, Mulders DG, Haagmans BL, van der Veer B, van den Brink S, Wijsman L, Goderski G, Romette JL, Ellis J, Zambon M, Peiris M, Goossens H, Reusken C, Koopmans MP, Drosten C. Corman VM, et al. Euro Surveill. 2020 Jan;25(3):2000045. doi: 10.2807/1560-7917.ES.2020.25.3.2000045. Euro Surveill. 2020. PMID: 31992387 Free PMC article.

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