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. 2020 Jun 15:196:110533.
doi: 10.1016/j.ecoenv.2020.110533. Epub 2020 Apr 1.

Production of a specific monoclonal antibody for 1-naphthol based on novel hapten strategy and development of an easy-to-use ELISA in urine samples

Zi-Jian Chen  1 , Xi-Xia Liu  2 , Zhi-Li Xiao  3 , Hui-Jun Fu  4 , Yu-Ping Huang  5 , Shu-Yi Huang  6 , Yu-Dong Shen  7 , Fan He  8 , Xing-Xing Yang  9 , Bruce Hammock  10 , Zhen-Lin Xu  11
Affiliations

Production of a specific monoclonal antibody for 1-naphthol based on novel hapten strategy and development of an easy-to-use ELISA in urine samples

Zi-Jian Chen et al. Ecotoxicol Environ Saf. .

Abstract

1-naphthol (1-NAP) is the main metabolite of pesticide carbaryl and naphthalene, and is also a genotoxic and carcinogenic intermediate in the synthesis of organic compound, dyes, pigment and pharmaceutical industry. In this work, two novel haptens were designed and synthesized for developing a competitive indirect enzyme-linked immunosorbent assay (ciELISA) method for 1-NAP in urine samples. The assay showed a limit of detection of 2.21 ng/mL and working range from 4.02 ng/mL to 31.25 ng/mL for 1-NAP in optimized working buffer. The matrix effect of samples was eliminated via 15-fold dilution of optimized working buffer. Good average recoveries (102.4%-123.4%) with a coefficient of variation from 11.7% to 14.7% was obtained for spiked urine samples. Subsequent instrument verification test showed good correlation between the results of ciELISA and high-performance liquid chromatography. The developed ciELISA is a high-throughput tool to monitor 1-NAP in urine, which can provide technical support for the establishment of biological exposure level for the exposure to carbaryl, naphthalene and other related pollutants.

Keywords: 1-naphthol; Hapten design; Immunoassay; Monoclonal antibody.

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Conflict of interest statement

Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Figures

Fig. 1
Fig. 1
The schematic of developed ciELISA for 1-NAP.
Fig. 2
Fig. 2
Comparison of surface electrostatic potential image of 1-NAP haptens between this study and previous study (Kramer et al., 1994).
Fig. 3
Fig. 3
Calibration curve (A) and ciELISA (B) calibration curve for determination of 1-NAP in optimized working buffer (n=6).
Fig. 4
Fig. 4
Matrix effect of urine samples on ciELISA performance (n=6).
See this image and copyright information in PMC

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