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. 1988 Nov 30;71(2):315-21.
doi: 10.1016/0378-1119(88)90048-0.

Complete nucleotide sequence of pAL5000, a plasmid from Mycobacterium fortuitum

J Rauzier  1 , J Moniz-Pereira, B Gicquel-Sanzey
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Complete nucleotide sequence of pAL5000, a plasmid from Mycobacterium fortuitum

J Rauzier et al. Gene. .

Abstract

We have determined the complete nucleotide sequence of pAL5000, a plasmid from Mycobacterium fortuitum; the plasmid contains 4837 bp with 65% G + C. Five open reading frames (ORF1 to ORF5) have been identified. A number of sequences corresponding to palindromes, repeats, a helix-turn-helix motif, a signal sequence and repetitive amino acid motifs can be identified. This sequence should facilitate the construction of vectors based on pAL5000 for transfer and expression studies in mycobacteria.

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