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Review
. 2020 Aug 1;12(8):569-579.
doi: 10.1093/jmcb/mjaa012.

Organoid technology for tissue engineering

Affiliations
Review

Organoid technology for tissue engineering

Juan He et al. J Mol Cell Biol. .

Abstract

For centuries, attempts have been continuously made to artificially reconstitute counterparts of in vivo organs from their tissues or cells. Only in the recent decade has organoid technology as a whole technological field systematically emerged and been shown to play important roles in tissue engineering. Based on their self-organizing capacities, stem cells of versatile organs, both harvested and induced, can form 3D structures that are structurally and functionally similar to their in vivo counterparts. These organoid models provide a powerful platform for elucidating the development mechanisms, modeling diseases, and screening drug candidates. In this review, we will summarize the advances of this technology for generating various organoids of tissues from the three germ layers and discuss their drawbacks and prospects for tissue engineering.

Keywords: 3D culture; organoid; tissue engineering.

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Figures

Figure 1
Figure 1
The versatile organoids generated from hPSCs. The organoids for organs in the three germ layers can be derived from iPSCs. PSCs could be induced to form the three germ layers, including the definitive endoderm, mesoderm, and ectoderm. The three germ layers could be further differentiated into various organs, such as the esophagus, lung, liver, pancreas, gut, stomach, and prostate for definitive endoderm, renal and blood vessels for mesoderm, and brain and skin for ectoderm.

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References

    1. Adams, E.J., Karthaus, W.R., Hoover, E., et al. (2019). FOXA1 mutations alter pioneering activity, differentiation and prostate cancer phenotypes. Nature 571, 408–412. - PMC - PubMed
    1. Amiri, A., Coppola, G., Scuderi, S., et al. (2018). Transcriptome and epigenome landscape of human cortical development modeled in organoids. Science 362, eaat6720. - PMC - PubMed
    1. Artegiani, B., van Voorthuijsen, L., Lindeboom, R.G.H., et al. (2019). Probing the tumor suppressor function of BAP1 in CRISPR-engineered human liver organoids. Cell Stem Cell 24, 927–943.e6. - PubMed
    1. Bagley, J.A., Reumann, D., Bian, S., et al. (2017). Fused cerebral organoids model interactions between brain regions. Nat. Methods 14, 743–751. - PMC - PubMed
    1. Balakrishnan, B., and Banerjee, R. (2011). Biopolymer-based hydrogels for cartilage tissue engineering. Chem. Rev. 111, 4453–4474. - PubMed

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