Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2020 Jun;60(2):196-205.
doi: 10.1007/s12088-019-00850-w. Epub 2019 Dec 11.

Respiratory Bacterial Microbiota and Individual Bacterial Variability in Lung Cancer and Bronchiectasis Patients

Anuradha Ekanayake  1 , Dushantha Madegedara  2 , Vishvanath Chandrasekharan  3 , Dhammika Magana-Arachchi  1
Affiliations

Respiratory Bacterial Microbiota and Individual Bacterial Variability in Lung Cancer and Bronchiectasis Patients

Anuradha Ekanayake et al. Indian J Microbiol. 2020 Jun.

Abstract

Respiratory bacterial microbiota plays a key role in human health. Lung cancer microbiome is a significant yet an understudied area while bronchiectasis microbiome is often studied. We assessed the bacterial microbiota in the upper and lower respiratory tract of the patients with lung cancer and bronchiectasis against a healthy group and their variations in individuality. 16S rRNA gene based metagenomic sequencing was used to detect entire bacterial community along with conventional aerobic bacterial culturing. In comparison to healthy, increased bacterial diversity was observed in diseased population. Abundance of more than 1% was considered and bacteria were identified in 97% similarity. Only lung cancer patients exhibited bacteria specific to the disease: Corynebacterium tuberculostearicum and Keratinibaculum paraultunense. However, Enterococcus faecalis and Delftia tsuruhatensis were also observed limited to lung cancer and bronchiectasis respectively, in less than 1% but supported with bacterial culturing. In conclusion the disease condition and intra-group variability should be considered in future with larger cohorts to understand individual patient variability highlighting the social habits and gender of the individual.

Keywords: 16S metagenomics; Bacteria; Bronchiectasis; Lung cancer; Lung microbiota; Respiratory tract.

PubMed Disclaimer

Conflict of interest statement

Conflict of interestThe author(s) declare no competing interests.

Figures

Fig. 1
Fig. 1
Shannon and Simpson diversity variation of the bacterial diversity in the three groups. a Healthy population. b Lung cancer population. c Bronchiectasis population
Fig. 2
Fig. 2
Bacterial taxa summary according to the phylum. a Healthy population. b Lung cancer population. c Bronchiectasis population. HV healthy volunteers, LC lung cancer, BR bronchiectasis
Fig. 3
Fig. 3
Phylogenetic tree of the bacterial species identified by culture method using the maximum likelihood method. Reliability for internal branch was assessed using the aLRT test (SH-Like). Leaves are colour coded representing different organism. Heatmap gradient represent the identified number of strains
See this image and copyright information in PMC

References

    1. Lederberg J, McCray JE (2001) ‘Ome Sweet’ Omics—a genealogical treasury of words | The Scientist Magazine®. In: Sci. https://www.the-scientist.com/commentary/ome-sweet-omics—a-genealogical-.... Accessed 7 March 2019
    1. Young RP, Hopkins RJ, Christmas T, et al. COPD prevalence is increased in lung cancer, independent of age, sex and smoking history. Eur Respir J. 2009;34:380–386. doi: 10.1183/09031936.00144208. - DOI - PubMed
    1. Yu Y-H, Liao C-C, Hsu W-H, et al. Increased lung cancer risk among patients with pulmonary tuberculosis: a population cohort study. J Thorac Oncol. 2011;6:32–37. doi: 10.1097/JTO.0b013e3181fb4fcc. - DOI - PubMed
    1. Wu BG, Cahaney CF, Tsay JJ et al (2015) C99 genomics and cancer: has it borne scientific and clinical fruit? Evaluation of the microbiome associated with lung cancer. In: American Thoracic Society 2015 international conference
    1. Hosgood HD, Sapkota AR, Rothman N, et al. The potential role of lung microbiota in lung cancer attributed to household coal burning exposures. Environ Mol Mutagen. 2014;55:643–651. doi: 10.1002/em.21878. - DOI - PMC - PubMed