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. 2020 Feb 21;6(4):FSO459.
doi: 10.2144/fsoa-2019-0111.

Automated imaging cytometry reveals dysplastic indices of colonic serrated adenomas

Nicholas S Samel  1 , Qin Huang  2 , Hiroshi Mashimo  2
Affiliations

Automated imaging cytometry reveals dysplastic indices of colonic serrated adenomas

Nicholas S Samel et al. Future Sci OA. .

Abstract

Aim: Left-sided colonic serrated adenomas (L-SAs) were evaluated for aneuploidy using automated imaging cytometry to quantify DNA content and compared with normal colonic tissues (NCT), tubular adenomas (TA), left-sided hyperplastic polyps (L-HP) and adenocarcinomas.

Materials & methods: We used standard paraffin-embedded Feulgen-stained tissue sections.

Results: The mean DNA index (DI) of NCT was 0.95, L-HP was 1.08, TA was 1.22, L-SA was 1.11 and adenocarcinomas was 1.46. DI of L-SA was statistically higher than that of NCT, but not statistically different from L-HP.

Conclusion: This study demonstrates that DIs correlate with the described neoplastic progression of L-SA, TA and L-SA compared with NCT and suggests that L-SA may be involved in a chromosome instability pathway of neoplastic progression.

Keywords: Feulgen stain; colon polyps; dysplasia; image cytometry; serrated adenoma.

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Conflict of interest statement

Financial & competing interests disclosure The authors have no relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript. This includes employment, consultancies, honoraria, stock ownership or options, expert testimony, grants or patents received or pending, or royalties. No writing assistance was utilized in the production of this manuscript.

Figures

Figure 1.
Figure 1.. Examples of encircled nuclei of Feulgen-stained epithelial cells to derive DNA index histograms for normal colonic tissue, left-sided serrated adenoma, tubular adenoma and adenocarcinoma samples.
A line representing DNA index of 1 is derived from the modal integrated optical density from encircling nuclei of lymphocytes on the same slide. The scale bars on the upper images represent about 400 μm and the scale bars on the lower images represent about 40 μm. AC: Adenocarcinoma; L-SA: Left-sided serrated adenoma; NCT: Normal colonic tissue; TA: Tubular adenoma.
Figure 2.
Figure 2.. DNA index comparison of normal colonic tissue, left-sided hyperplastic polyp, left-sided serrated adenoma, tubular adenoma and adenocarcinoma.
*Indicates statistical significance in DI from normal colonic tissue. AC: Adenocarcinoma; DI: DNA index; L-HP: Left-sided hyperplastic polyp; L-SA: Left-sided serrated adenoma; TA: Tubular adenoma.
Figure 3.
Figure 3.. Comparing flow cytometry (from literature) and automated imaging cytometry in terms of the resulting percent of samples with aneuploidy for each type of colonic lesion.
AC: Adenocarcinoma; AIC: Automated imaging cytometry; FC: Flow cytometry; HP: Hyperplastic polyp; SA: Serrated adenoma; TA: Tubular adenoma.
See this image and copyright information in PMC

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