Reference values of hematological and biochemical parameters in young-adult cynomolgus monkey (Macaca fascicularis) and rhesus monkey (Macaca mulatta) anesthetized with ketamine hydrochloride

Bon-Sang Koo^#¹, Dong-Ho Lee^#², Philyong Kang³, Kang-Jin Jeong¹, Sangil Lee², Kijin Kim², Youngjeon Lee^{1

4}, Jae-Won Huh^{1

4}, Young-Hyun Kim^{1

4}, Sang-Je Park¹, Yeung Bae Jin¹, Sun-Uk Kim^{3

4}, Ji-Su Kim^{2

4}, Yeonghoon Son², Sang-Rae Lee^{1

4}

Affiliations

¹ 2National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology, 30 Yeongudanji-ro, Ochang-eup, Chungwon-gu, Cheongju-si, Chungbuk 28116 Republic of Korea.
² 1Primate Resources Center, Korea Research Institute of Bioscience and Biotechnology, 351-33, Neongme-gil, Ibam-myeon, Jeongup-si, Jeonbuk 56216 Republic of Korea.
³ 3Futuristic Animal Resource & Research Center, Korea Research Institute of Bioscience and Biotechnology, Chungbuk, 28116 Republic of Korea.
⁴ 4Department of Functional Genomics, University of Science and Technology, Daejeon, 34113 Republic of Korea.

^# Contributed equally.

PMID: 32257895
PMCID: PMC7081622
DOI: 10.1186/s42826-019-0006-0

Reference values of hematological and biochemical parameters in young-adult cynomolgus monkey (Macaca fascicularis) and rhesus monkey (Macaca mulatta) anesthetized with ketamine hydrochloride

Bon-Sang Koo et al. Lab Anim Res. 2019.

. 2019 Jul 24:35:7.

doi: 10.1186/s42826-019-0006-0. eCollection 2019.

Authors

Affiliations

¹ 2National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology, 30 Yeongudanji-ro, Ochang-eup, Chungwon-gu, Cheongju-si, Chungbuk 28116 Republic of Korea.
² 1Primate Resources Center, Korea Research Institute of Bioscience and Biotechnology, 351-33, Neongme-gil, Ibam-myeon, Jeongup-si, Jeonbuk 56216 Republic of Korea.
³ 3Futuristic Animal Resource & Research Center, Korea Research Institute of Bioscience and Biotechnology, Chungbuk, 28116 Republic of Korea.
⁴ 4Department of Functional Genomics, University of Science and Technology, Daejeon, 34113 Republic of Korea.

^# Contributed equally.

PMID: 32257895
PMCID: PMC7081622
DOI: 10.1186/s42826-019-0006-0

Abstract

Nonhuman primate models are valuable in biomedical research. However, reference data for clinical pathology parameters in cynomolgus and rhesus monkeys are limited. In the present study, we established hematologic and biochemical reference intervals for healthy cynomolgus and rhesus monkeys anesthetized with ketamine hydrochloride. A total of 142 cynomolgus monkeys (28 males and 114 females) and 42 rhesus monkeys (22 males and 20 females) were selected and analyzed in order to examine reference intervals of 20 hematological and 16 biochemical parameters. The effects of sex were also investigated. Reference intervals for hematological and biochemical parameters were separately established by species (cynomolgus and rhesus) and sex (male and female). No sex-related differences were determined in erythrocyte-related parameters for cynomolgus and rhesus monkey housed in indoor laboratory conditions. Alkaline phosphatase and gamma glutamyltransferase were significantly lower in females than males in both cynomolgus and rhesus monkeys aged 48-96 months. The reference values for hematological and biochemical parameters established herein might provide valuable information for researchers using cynomolgus and rhesus monkeys in experimental conditions for biomedical studies.

Keywords: Biochemistry; Cynomolgus monkey; Hematology; Non-human primate; Rhesus monkey.

PubMed Disclaimer

Conflict of interest statement

Competing interestsThe authors declare that they have no competing interests.

Cited by

Mayaro virus pathogenesis and immunity in rhesus macaques.
Weber WC, Labriola CS, Kreklywich CN, Ray K, Haese NN, Andoh TF, Denton M, Medica S, Streblow MM, Smith PP, Mizuno N, Frias N, Fisher MB, Barber-Axthelm AM, Chun K, Uttke S, Whitcomb D, DeFilippis V, Rakshe S, Fei SS, Axthelm MK, Smedley JV, Streblow DN. Weber WC, et al. PLoS Negl Trop Dis. 2023 Nov 20;17(11):e0011742. doi: 10.1371/journal.pntd.0011742. eCollection 2023 Nov. PLoS Negl Trop Dis. 2023. PMID: 37983245 Free PMC article.
Reference Intervals and Percentiles for Hematologic and Serum Biochemical Values in Captive Bred Rhesus (Macaca mulatta) and Cynomolgus Macaques (Macaca fascicularis).
Bakker J, Maaskant A, Wegman M, Zijlmans DGM, Hage P, Langermans JAM, Remarque EJ. Bakker J, et al. Animals (Basel). 2023 Jan 28;13(3):445. doi: 10.3390/ani13030445. Animals (Basel). 2023. PMID: 36766334 Free PMC article.
Blood Analysis of Laboratory Macaca mulatta Used for Neuroscience Research: Investigation of Long-Term and Cumulative Effects of Implants, Fluid Control, and Laboratory Procedures.
Wegener D, Oh 胡箪棋 DQP, Lukaß H, Böer M, Kreiter AK. Wegener D, et al. eNeuro. 2021 Oct 19;8(5):ENEURO.0284-21.2021. doi: 10.1523/ENEURO.0284-21.2021. Print 2021 Sep-Oct. eNeuro. 2021. PMID: 34556556 Free PMC article.
A modular approach toward producing nanotherapeutics targeting the innate immune system.
van Leent MMT, Meerwaldt AE, Berchouchi A, Toner YC, Burnett ME, Klein ED, Verschuur AVD, Nauta SA, Munitz J, Prévot G, van Leeuwen EM, Ordikhani F, Mourits VP, Calcagno C, Robson PM, Soultanidis G, Reiner T, Joosten RRM, Friedrich H, Madsen JC, Kluza E, van der Meel R, Joosten LAB, Netea MG, Ochando J, Fayad ZA, Pérez-Medina C, Mulder WJM, Teunissen AJP. van Leent MMT, et al. Sci Adv. 2021 Mar 5;7(10):eabe7853. doi: 10.1126/sciadv.abe7853. Print 2021 Mar. Sci Adv. 2021. PMID: 33674313 Free PMC article.
Dynamic Control Balancing Cell Proliferation and Inflammation is Crucial for an Effective Immune Response to Malaria.
Gupta A, Galinski MR, Voit EO. Gupta A, et al. Front Mol Biosci. 2022 Feb 15;8:800721. doi: 10.3389/fmolb.2021.800721. eCollection 2021. Front Mol Biosci. 2022. PMID: 35242812 Free PMC article.

See all "Cited by" articles

References

1. Gibbs RA, Weinstock GM, Metzker ML, Muzny DM, Sodergren EJ, Scherer S, Scott G, Steffen D, Worley KC, Burch PE, Okwuonu G, Hines S, Lewis L, DeRamo C, Delgado O, Dugan-Rocha S, Miner G, Morgan M, Hawes A, Gill R, Celera HRA, Adams MD, Amanatides PG, Baden-Tillson H, Barnstead M, Chin S, Evans CA, Ferriera S, Fosler C, Glodek A, Gu Z, Jennings D, Kraft CL, Nguyen T, Pfannkoch CM, Sitter C, Sutton GG, Venter JC, Woodage T, Smith D, Lee HM, Gustafson E, Cahill P, Kana A, Doucette-Stamm L, Weinstock K, Fechtel K, Weiss RB, Dunn DM, Green ED, Blakesley RW, Bouffard GG, De Jong PJ, Osoegawa K, Zhu B, Marra M, Schein J, Bosdet I, Fjell C, Jones S, Krzywinski M, Mathewson C, Siddiqui A, Wye N, McPherson J, Zhao S, Fraser CM, Shetty J, Shatsman S, Geer K, Chen Y, Abramzon S, Nierman WC, Havlak PH, Chen R, Durbin KJ, Egan A, Ren Y, Song XZ, Li B, Liu Y, Qin X, Cawley S, Worley KC, Cooney AJ, D’Souza LM, Martin K, Wu JQ, Gonzalez-Garay ML, Jackson AR, Kalafus KJ, McLeod MP, Milosavljevic A, Virk D, Volkov A, Wheeler DA, Zhang Z, Bailey JA, Eichler EE, Tuzun E, Birney E, Mongin E, Ureta-Vidal A, Woodwark C, Zdobnov E, Bork P, Suyama M, Torrents D, Alexandersson M, Trask BJ, Young JM, Huang H, Wang H, Xing H, Daniels S, Gietzen D, Schmidt J, Stevens K, Vitt U, Wingrove J, Camara F, Mar Alba M, Abril JF, Guigo R, Smit A, Dubchak I, Rubin EM, Couronne O, Poliakov A, Hubner N, Ganten D, Goesele C, Hummel O, Kreitler T, Lee YA, Monti J, Schulz H, Zimdahl H, Himmelbauer H, Lehrach H, Jacob HJ, Bromberg S, Gullings-Handley J, Jensen-Seaman MI, Kwitek AE, Lazar J, Pasko D, Tonellato PJ, Twigger S, Ponting CP, Duarte JM, Rice S, Goodstadt L, Beatson SA, Emes RD, Winter EE, Webber C, Brandt P, Nyakatura G, Adetobi M, Chiaromonte F, Elnitski L, Eswara P, Hardison RC, Hou M, Kolbe D, Makova K, Miller W, Nekrutenko A, Riemer C, Schwartz S, Taylor J, Yang S, Zhang Y, Lindpaintner K, Andrews TD, Caccamo M, Clamp M, Clarke L, Curwen V, Durbin R, Eyras E, Searle SM, Cooper GM, Batzoglou S, Brudno M, Sidow A, Stone EA, Venter JC, Payseur BA, Bourque G, Lopez-Otin C, Puente XS, Chakrabarti K, Chatterji S, Dewey C, Pachter L, Bray N, Yap VB, Caspi A, Tesler G, Pevzner PA, Haussler D, Roskin KM, Baertsch R, Clawson H, Furey TS, Hinrichs AS, Karolchik D, Kent WJ, Rosenbloom KR, Trumbower H, Weirauch M, Cooper DN, Stenson PD, Ma B, Brent M, Arumugam M, Shteynberg D, Copley RR, Taylor MS, Riethman H, Mudunuri U, Peterson J, Guyer M, Felsenfeld A, Old S, Mockrin S, Collins F, Rat Genome Sequencing Project C Genome sequence of the Brown Norway rat yields insights into mammalian evolution. Nature. 2004;428(6982):493–521. doi: 10.1038/nature02426. - DOI - PubMed
1. Grow DA, McCarrey JR, Navara CS. Advantages of nonhuman primates as preclinical models for evaluating stem cell-based therapies for Parkinson's disease. Stem Cell Res. 2016;17(2):352–366. doi: 10.1016/j.scr.2016.08.013. - DOI - PubMed
1. Lee JR, Park SJ, Kim YH, Choe SH, Cho HM, Lee SR, Kim SU, Kim JS, Sim BW, Song BS, Jeong KJ, Lee Y, Jin YB, Kang P, Huh JW, Chang KT. Alu-derived alternative splicing events specific to Macaca lineages in CTSF gene. Mol Cells. 2017;40(2):100–108. - PMC - PubMed
1. Yeo HG, Lee Y, Jeon CY, Jeong KJ, Jin YB, Kang P, Kim SU, Kim JS, Huh JW, Kim YH, Sim BW, Song BS, Park YH, Hong Y, Lee SR, Chang KT. Characterization of cerebral damage in a monkey model of Alzheimer's disease induced by Intracerebroventricular injection of Streptozotocin. J Alzheimers Dis. 2015;46(4):989–1005. doi: 10.3233/JAD-143222. - DOI - PubMed
1. Yi Kyung Sik, Choi Chi-Hoon, Lee Sang-Rae, Lee Hong Jun, Lee Youngjeon, Jeong Kang-Jin, Hwang Jinwoo, Chang Kyu-Tae, Cha Sang-Hoon. Sustained diffusion reversal with in-bore reperfusion in monkey stroke models: Confirmed by prospective magnetic resonance imaging. Journal of Cerebral Blood Flow & Metabolism. 2016;37(6):2002–2012. doi: 10.1177/0271678X16659302. - DOI - PMC - PubMed

LinkOut - more resources

Full Text Sources

Save citation to file

Email citation

Add to Collections

Add to My Bibliography

Your saved search

Create a file for external citation management software

Your RSS Feed

Reference values of hematological and biochemical parameters in young-adult cynomolgus monkey (Macaca fascicularis) and rhesus monkey (Macaca mulatta) anesthetized with ketamine hydrochloride

Affiliations

Reference values of hematological and biochemical parameters in young-adult cynomolgus monkey (Macaca fascicularis) and rhesus monkey (Macaca mulatta) anesthetized with ketamine hydrochloride

Authors

Affiliations

Abstract

Conflict of interest statement

Similar articles

Cited by

References

LinkOut - more resources

Full Text Sources