Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2020 Mar 24;64(1):73-78.
doi: 10.2478/jvetres-2020-0021. eCollection 2020 Mar.

Leptospira Interrogans Serogroup Sejroe Serovar Hardjo in Aborting Cows: Two Herd Cases in Sicily (Italy)

Francesca Grippi  1 , Elisabetta Giudice  2 , Simona Di Pietro  2 , Carmela Sciacca  1 , Francesco Santangelo  1 , Paola Galluzzo  1 , Santino Barreca  1 , Annalisa Guercio  1
Affiliations

Leptospira Interrogans Serogroup Sejroe Serovar Hardjo in Aborting Cows: Two Herd Cases in Sicily (Italy)

Francesca Grippi et al. J Vet Res. .

Abstract

Introduction: The aim of this study was to present two outbreaks of bovine abortion due to Leptospira infection in cattle herds located in the northern part of Sicily (Italy). The animals were positive for Leptospira interrogans serogroup Sejroe serovar Hardjo in a microscopic agglutination test (MAT).

Material and methods: A total of 23 Charolaise cows (farm A) and 75 Limousine bulls and Cinisara and Modicana cows (farm B) were enrolled in this study. The blood samples were collected from all subjects at the following time points: before a cycle of intramuscular treatment with oxytetracycline dihydrate (T0), after 5-6 weeks from the treatment (T1), and every 10 weeks until seronegativisation (T2 in Farm A and T3 in Farm B). A serological test (MAT) was used for the diagnosis of leptospirosis.

Results: Two samples from farm A (2/23) and 29 samples from farm B (29/75) were positive to Leptospira interrogans, serogroup Sejroe, serovar Hardjo in the MAT. Leptospira spp. DNA was detected by real-time PCR in the urine sample of one positive cow on farm A, and in placenta and brain samples belonging to one aborted foetus on farm B.

Conclusion: It is important to use serological and molecular diagnostic techniques complementarily to identify infected individuals.

Keywords: PCR; cattle; leptospirosis; microscopic agglutination test; zoonosis.

PubMed Disclaimer

Conflict of interest statement

Conflict of Interest Conflict of Interest Statement: The authors declare that there is no conflict of interests regarding the publication of this article.

Figures

Fig. 1
Fig. 1
Geographical area where the farms are located
See this image and copyright information in PMC

References

    1. Adugna S.A.. Review of bovine leptospirosis. Eur J Appl Sci. 2016;8:347–355.
    1. Alt D.P., Zuerner R.L., Bolin C.A.. Evaluation of antibiotics for treatment of cattle infected with Leptospira borgpetersenii serovar Hardjo. J Am Vet Med Assoc. 2001;219:636–639. - PubMed
    1. Autorino G.L., Salvati F., Ammadeo D., Korver H., Ellis W.A. Identification of leptospires belonging to the Australis group isolated in central Italy. Proc. 8th Meeting of European Leptospira Workers Anzio. 1994. p. 15. July.
    1. Benschop J., Collins-Emerson J., Maskill A., O’Connor P., Tunbridge M., Yupiana Y., Weston J.. Leptospirosis in three workers on a dairy farm with unvaccinated cattle. N Z Med J. 2017;130:102–108. - PubMed
    1. Chiari M., Figarolli B.M., Tagliabue S., Alborali G.L., Bertoletti M., Papetti A., D’Incau M., Zanoni M., Boniotti M.B.. Seroprevalence and risk factors of leptospirosis in wild boars Sus scrofa in northern Italy. Hystrix It J Mamm. 2016;27 doi: 10.4404/hystrix-27.2-11682. - DOI

LinkOut - more resources