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. 2015 Jan 21;3(3):348-352.
doi: 10.1039/c4tb01569j. Epub 2014 Dec 3.

A G-triplex luminescent switch-on probe for the detection of mung bean nuclease activity

Dik-Lung Ma  1 , Lihua Lu, Sheng Lin, Bingyong He, Chung-Hang Leung
Affiliations

A G-triplex luminescent switch-on probe for the detection of mung bean nuclease activity

Dik-Lung Ma et al. J Mater Chem B. .

Abstract

A cyclometallated Ir(iii) complex was synthesised and evaluated for the ability to act as a luminescent G-triplex probe. The iridium(iii) complex 1 [Ir(phq)2(2,9-dmphen)]PF6 (where phq = 2-phenylquinoline; 2,9-dmphen = 2,9-dimethyl-1,10-phenanthroline) exhibited high luminescence for G-triplex DNA over dsDNA and ssDNA, and was employed to construct a label-free G-triplex-based assay for mung bean nuclease activity in aqueous solution. This method was highly sensitive and selective for MB nuclease activity over other DNA-modifying enzymes.

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