Loading and release of fluorescent oligoarginine peptides into/from pH-responsive anionic supramolecular nanoparticles

Abstract

Supramolecular nanoparticles (SNPs) based on negatively charged polymeric components can act as pH-responsive systems which allow the encapsulation and release of a positively charged cargo by electrostatic interactions. Fluorescent SNPs, based on the negatively charged poly(isobutyl-alt-maleic acid) and labeled with rhodamine B, were used as carriers to encapsulate positively charged Arg_n peptides grafted with a cyanine dye. The energy transfer (FRET) between the dyes residing in a single particle was used to provide a sensing mechanism to study the encapsulation and release of the peptide cargo into/from the SNPs. The change in the spectral signature of the cyanine dye from encapsulated in the SNPs to free in solution was used to characterize the Arg_n release. Finally, in vitro experiments revealed that the Arg_n release from these SNPs occurred at the pH drop that mimics lysosome conditions.