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. 1988:(19):117-20.

A new method for the unidirectional deletion of DNA with Bal 31 nuclease using 2'-O-MeRNA-DNA chimeric adaptors

S Mukai  1 , S Shibahara, H Morisawa
Affiliations
  • PMID: 3226906

A new method for the unidirectional deletion of DNA with Bal 31 nuclease using 2'-O-MeRNA-DNA chimeric adaptors

S Mukai et al. Nucleic Acids Symp Ser. 1988.

Abstract

We have developed a new procedure for unidirectional DNA deletion with Bal 31 nuclease. This method is based on the use of chimeric adaptors containing double strands of 2'-O-methyl RNA to block against an attack of Bal 31 nuclease. This contributes a wide application to learn the relation of structure-function of genes.

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