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. 2020 Jul;52(6):e13579.
doi: 10.1111/and.13579. Epub 2020 Apr 9.

Association between seminal granulysin and malondialdehyde in infertile men with varicocele and the potential effect of varicocelectomy

Howyda M Kamal  1 , Asmaa A El-Fallah  1 , Shabieb A Abdelbaki  2 , Mostafa M Khalil  2 , Mai M Kamal  3 , Eman G Behiry  1
Affiliations

Association between seminal granulysin and malondialdehyde in infertile men with varicocele and the potential effect of varicocelectomy

Howyda M Kamal et al. Andrologia. 2020 Jul.

Abstract

This study assessed the seminal plasma granulysin and malondialdehyde (MDA) levels in patients suffering from varicocele-associated infertility prior to and after varicocelectomy. This study was conducted on 34 infertile men with varicocele (group A) and same patients after varicocelectomy (group B) and 32 fertile normozoospermic males (group C). A detailed history taking, clinical examination, scrotal doppler ultrasound for varicocele diagnosis and grading, semen analysis and estimation of seminal granulysin and MDA before and after varicocelectomy were done to all participants. The mean (SD) granulysin and MDA levels in patients with varicocele were higher than in controls with highly significant differences. Post-operatively, there was a significant reduction in mean (SD) granulysin and in MDA level. Basal seminal granulysin positively correlated with basal seminal MDA, abnormal forms and negatively correlated with basal sperm count, concentration, and progressive motility. The receiver operating characteristic curve of seminal granulysin and MDA levels were conducted for discrimination between infertility cases with varicocele and control groups. Excellent AUCs were found for both markers (AUC = 0.971, 0.991 respectively). We concluded that high levels of granulysin and MDA in the semen of infertile males with varicocele negatively impact their spermatogenesis. Varicocelectomy leads to the improvement of semen parameters and significantly decreases seminal plasma granulysin and MDA levels. Hence, seminal granulysin and MDA could be used as a prognostic test in infertile patients with varicocele.

Keywords: granulysin; male infertility; oxidative stress; varicocelectomy.

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References

REFERENCES

    1. Agarwal, A., Virk, G., Ong, C., & Du Plessis, S. S. (2014). Effect of oxidative stress on male reproduction. The World Journal of Men's Health, 32(1), 1-17.
    1. Alsaikhan, B., Alrabeeah, K., Delouya, G., & Zini, A. (2016). Epidemiology of varicocele. Asian Journal of Andrology, 18(2), 179.
    1. Anderson, D. H., Sawaya, M. R., Cascio, D., Ernst, W., Modlin, R., Krensky, A., & Eisenberg, D. (2003). Granulysin crystal structure and a structure-derived lytic mechanism. Journal of Molecular Biology, 325(2), 355-365.
    1. Aporta, A., Catalán, E., Galán-Malo, P., Ramírez-Labrada, A., Pérez, M., Azaceta, G., … Anel, A. (2014). Granulysin induces apoptotic cell death and cleavage of the autophagy regulator Atg5 in human hematological tumors. Biochemical Pharmacology, 87(3), 410-423.
    1. Chen, C.-H., Lee, S.-S., Chen, D.-C., Chien, H.-H., Chen, I.-C., Chu, Y.-N., … Wu, G.-J. (2004). Apoptosis and kinematics of ejaculated spermatozoa in patients with varicocele. Journal of Andrology, 25(3), 348-353.

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