Influence of muscle oxygenation and nitrate-rich beetroot juice supplementation on O2 uptake kinetics and exercise tolerance

Abstract

We tested the hypothesis that acute supplementation with nitrate (NO₃^-)-rich beetroot juice (BR) would improve quadriceps muscle oxygenation, pulmonary oxygen uptake (V˙O₂) kinetics and exercise tolerance (T_lim) in normoxia and that these improvements would be augmented in hypoxia and attenuated in hyperoxia. In a randomised, double-blind, cross-over study, ten healthy males completed two-step cycle tests to T_lim following acute consumption of 210 mL BR (18.6 mmol NO₃^-) or NO₃^--depleted beetroot juice placebo (PL; 0.12 mmol NO₃^-). These tests were completed in normobaric normoxia [fraction of inspired oxygen (FIO₂): 21%], hypoxia (FIO₂: 15%) and hyperoxia (FIO₂: 40%). Pulmonary V˙O₂ and quadriceps tissue oxygenation index (TOI), derived from multi-channel near-infrared spectroscopy, were measured during all trials. Plasma [nitrite] was higher in all BR compared to all PL trials (P < 0.05). Quadriceps TOI was higher in normoxia compared to hypoxia (P < 0.05) and higher in hyperoxia compared to hypoxia and normoxia (P < 0.05). T_lim was improved after BR compared to PL ingestion in the hypoxic trials (250 ± 44 vs. 231 ± 41 s; P = 0.006; d = 1.13), with the magnitude of improvement being negatively correlated with quadriceps TOI at T_lim (r = -0.78; P < 0.05). T_lim was not improved following BR ingestion in normoxia (BR: 364 ± 98 vs. PL: 344 ± 78 s; P = 0.087, d = 0.61) or hyperoxia (BR: 492 ± 212 vs. PL: 472 ± 196 s; P = 0.273, d = 0.37). BR ingestion increased peak V˙O₂ in hypoxia (P < 0.05), but not normoxia or hyperoxia (P > 0.05). These findings indicate that BR supplementation is more likely to improve T_lim and peak V˙O₂ in situations when skeletal muscle is more hypoxic.

Keywords: Exercise performance; Fatigue; Near-infrared spectroscopy; Nitric oxide; Oxidative metabolism; Vascular function.