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. 2020 Mar 2;10(1):18-26.
doi: 10.18683/germs.2020.1181. eCollection 2020 Mar.

First report of VIM metallo-β-lactamase production in Escherichia coli and Klebsiella pneumoniae clinical isolates from Gaza Strip, Palestine

Ghassan Tayh  1 , Daram Nagarjuna  2 , Rym Ben Sallem  1 , Vivek Verma  2 , Sarra Chairat  3 , Abdellatif Boudabous  1 , Manisha Yadav  2 , Karim Ben Slama  1
Affiliations

First report of VIM metallo-β-lactamase production in Escherichia coli and Klebsiella pneumoniae clinical isolates from Gaza Strip, Palestine

Ghassan Tayh et al. Germs. .

Abstract

Introduction: Even though the increasing incidence of VIM-producing E. coli and K. pneumoniae has been reported worldwide, studies are still lacking in Palestine. The aim of this study was to screen carbapenem-resistant E. coli and K. pneumoniae bacteria in the Gaza Strip, Palestine and further to characterize carbapenemase-producing isolates.

Methods: A total of 69 E. coli and 27 K. pneumoniae isolates were obtained from three Gaza hospitals and recovered from urine, wound swabs, blood and ear discharge. The screening for metallo-β-lactamases (MBLs) was performed by using the imipenem-EDTA disc synergy test. The detection of β-lactamases genes, detection of non-β-lactam genes and the characterization of integrons were performed by PCR and sequencing. The clonal relationship among the isolates was determined by pulsed-field gel electrophoresis (PFGE).

Results: Our study showed that 4 E. coli (5.8%) and 5 K. pneumoniae (18.5%) were positive by the imipenem-EDTA disc synergy test. Bla VIM-4 was detected in six isolates and bla VIM-28 was identified in three isolates. The β-lactamases genes in the VIM-producing K. pneumoniae isolates were bla CTX-M-15 (n=3), bla CTX-M-14 (n=1), bla SHV-1 (n=3), bla SHV-12 (n=1), bla TEM-1 (n=1) and bla OXA-1 (n=1). Aac(6')-Ib-cr gene was confirmed in four E. coli and in two K. pneumoniae isolates. QnrS1 was identified in two K. pneumoniae isolates. The class 1 integron was identified with the different gene cassette; dfrA17-aadA5, dfrA5, dfrA12-orf-aadA2 and dfrA17-aadA5 were identified.

Conclusions: Our study indicated for the first time the emergence of multidrug-resistant VIM-containing K. pneumoniae and E. coli isolates of clinical origin in Gaza Strip hospitals.

Keywords: CTX-M ESBL; Palestine; VIM metallo-β-lactamase; antimicrobial resistance; carbapenem; integron.

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Conflict of interest statement

Conflicts of interest: All authors – none to declare.

Figures

Figure 1
Figure 1. Pulsed-field gel electrophoresis (PFGE) dendrogram rooted from XbaI-digested E. coli strains
Figure 2
Figure 2. Pulsed-field gel electrophoresis (PFGE) dendrogram rooted from XbaI-digested K. pneumoniae strains
See this image and copyright information in PMC

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