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. 2021 Apr;68(2):297-306.
doi: 10.1002/bab.1926. Epub 2020 May 22.

Purification and properties of a laccase from the mushroom Agaricus sinodeliciosus

Jingzhe Hao  1   2 , Weiwei Zhang  3 , Hexiang Wang  2 , Nur Ziya  1 , Ying Luo  1 , Peisong Jia  1 , Guoqing Zhang  4 , Tzibun Ng  5
Affiliations

Purification and properties of a laccase from the mushroom Agaricus sinodeliciosus

Jingzhe Hao et al. Biotechnol Appl Biochem. 2021 Apr.

Abstract

A homogeneous monomeric laccase (ASL) from Agaricus sinodeliciosus, with a molecular mass of 65 kDa, was isolated using ion-exchange chromatography (CM-cellulose and Q-Sepharose) and gel-filtration chromatography (Superdex 75). This laccase exhibited maximum activity at 50 °C and pH 5.0. Hg2+ and Cd2+ significantly inhibited its activity. The laccase displayed a Km value of 0.9 mM toward 2,2'-azinobis-(3-ethylbenzthiazoline-6-sulfonate) (ABTS). In addition to ABTS, ASL exhibited higher affinity toward o-toluidine and benzidine than other substrates. ASL is able to decolorize malachite green and Eriochrome black T.

Keywords: Agaricus sinodeliciosus; enzymatic properties; laccase; purification.

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References

    1. Parra, L. A., Callac, P., Zhou, J. L., Fu, W. J., Dui, S. H., Hyde, K. D., Zhao, R. L. (2015) Phytotaxa 202, 185-197.
    1. Zhou, J., Bai, X., Zhao, R. (2017) Sci. Rep. 7, 15719.
    1. Rivera-Hoyos, C. M., Morales-Alvarez, E. D., Poutou-Pinales, R. A., Pedroza-Rodriguez, A. M., Rodriguez-Vazquez, R., Delgado-Boada, J. M. (2013) Fungal Biol. Rev. 27, 67-82.
    1. Dwivedi, U. N., Singh, P., Pandey, V. P., Kumar, A. (2011) J. Mol. Catal. B Enzym. 68, 117-128.
    1. Shraddha , Shekher, R., Sehgal, S., Kamthania, M., Kumar, A. (2011) Enzyme Res. 2011, 217861.

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