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. 1987 Jul-Sep;138(3):307-314.
doi: 10.1016/S0769-2617(87)80017-5. Epub 2009 Sep 23.

Sequential variation in genomic RNA patterns of human rotaviruses isolated from infantile gastroenteritis

F J Buesa  1 , M Duato  1 , C Gimeno  1 , J García de Lomas  1
Affiliations

Sequential variation in genomic RNA patterns of human rotaviruses isolated from infantile gastroenteritis

F J Buesa et al. Ann Inst Pasteur Virol. 1987 Jul-Sep.

Abstract
in English, French

The incidence and RNA electrophoretypes of rotaviral isolates obtained from infants and young children with acute gastroenteritis were studied from October, 1985 through April, 1986. Analysis of the viral RNA was carried out by Polyacrylamide gel electrophoresis followed by silver staining. Fourteen electrophoretypes were identified. A single dominant electrophoretype was found during the first months of the rotavirus seasonal outbreak. In contrast, a large variety of RNA patterns were observed during the latter portion of the study period. Subgrouping of rotavirus isolates by a double-sandwich enzyme-linked immunosorbent assay using monoclonal-detecting antibodies showed that all strains belonged to subgroup II. Mixed rotavirus electrophoretypes appeared in 4 cases (8.16 %).

On a étudié l’incidence et les profils électrophorétiques de l’ARN génomique des souches de rotavirus obtenues d’octobre 1985 à avril 1986, de gastroentérites aiguës de l’enfant. L’analyse de l’ARN par électrophorèse sur gel de polyacrylamide a permis l’identification de 14 électrophorétypes différents. Pendant les premiers mois de la période d’étude, on a détecté un électrophorétype dominant; au contraire, dans les mois suivants, on a observé une grande variété de profils électrophorétiques.La détermination des sous-groupes des rotavirus par analyse immunoenzy-matique avec des anticorps monoclonaux détecteurs, a permis de constater que toutes les souches sont du sous-groupe II. Dans 4 échantillons (8.16 %) on a détecté des électrophorétypes mixtes.

Keywords: Molecular epidemiology, Genomic RNA patterns, Sequential variation, Electrophoretypes, Children; Rotavirus, RNA, Gastroenteritis.

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