Characterization of pro-opiomelanocortin cDNA from the Old World monkey, Macaca nemestrina

Abstract

An observation from high-pressure liquid chromatography (HPLC) suggesting that monkey beta-endorphin (BE) was chemically different from human or rat BE was investigated by determining the cDNA sequence for the monkey pro-opiomelanocortin (POMC) precursor. A full-length cDNA for POMC was isolated from a Macaca nemestrina whole pituitary cDNA library. The longest open reading frame predicts a 264-residue polypeptide exhibiting the basic structure of POMC that is closely homologous to the human counterpart. The monkey BE sequence apparently diverged from the human sequence after the latter had made the His-27 to Tyr-27 change but prior to the Gln-31 to Glu-31 transition, leaving it more hydrophobic than rat or human BE, consistent with its chromatography on reverse-phase HPLC. Comparison of the monkey POMC precursor with those of other species highlights conserved domains, presumably reflecting regions of physiological activity that await elucidation.