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. 2020 Jul;26(7):1633-1635.
doi: 10.3201/eid2607.201285. Epub 2020 Jun 21.

Triplex Real-Time RT-PCR for Severe Acute Respiratory Syndrome Coronavirus 2

Jesse J Waggoner, Victoria Stittleburg, Renee Pond, Youssef Saklawi, Malaya K Sahoo, Ahmed Babiker, Laila Hussaini, Colleen S Kraft, Benjamin A Pinsky, Evan J Anderson, Nadine Rouphael

Triplex Real-Time RT-PCR for Severe Acute Respiratory Syndrome Coronavirus 2

Jesse J Waggoner et al. Emerg Infect Dis. 2020 Jul.

Abstract

Most reverse transcription PCR protocols for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) include 2-3 targets for detection. We developed a triplex, real-time reverse transcription PCR for SARS-CoV-2 that maintained clinical performance compared with singleplex assays. This protocol could streamline detection and decrease reagent use during current high SARS-CoV-2 testing demands.

Keywords: 2019 novel coronavirus disease; COVID-19; SARS; SARS-CoV-2; coronavirus; coronavirus disease; molecular detection; rRT-PCR; respiratory infections; severe acute respiratory syndrome coronavirus 2; viruses; zoonoses.

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