Structural characterization and antioxidant potential of a novel exopolysaccharide produced by Bacillus velezensis SN-1 from spontaneously fermented Da-Jiang

Abstract

The aim of this study was to characterize the exopolysaccharide (EPS) produced by Bacillus velezensis SN-1 (B. velezensis SN-1) (EPS-SN-1), which was isolated from the fermented Da jiang. The microbe made crude exopolysaccharides EPS-SN-1 was produced throughout the bacterial growth period, and the highest yield (2.7 g/L) was obtained with sucrose as the carbon source. As per high performance liquid chromatography (HPLC), EPS-SN-1 is a heteropolysaccharide consisting of glucose, mannose and fructose, with a high molecular weight of 2.21 × 10⁵ Da. FTIR spectra further indicated the presence of hydroxyl and carbonyl groups, and NMR analysis confirmed both α- and β-glycosidic bonds. Furthermore, differential scanning calorimetry (DSC) showed that EPS-SN-1 has high thermal stability with fusion point of 270.7 °C. Finally, EPS-SN-1 demonstrated strong antioxidant capacity via its ability to scavenge hydroxyl radical (^•OH), 1,1-diphenyl-2-picrylhydrazyl (DPPH^•) radical, ABTS radical (ABTS^•+) and oxygen radical (O₂^-•). Taken together, EPS-SN-1 is a promising natural antioxidant and probiotic with potential applications in the food industry.

Keywords: Antioxidant activity; Bacillus velezensis SN-1; Characterization; Exopolysaccharide; Thermostability.