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Review
. 2020 Apr;85(4):760-768.
doi: 10.1002/cplu.202000167.

When Small becomes Too Big: Expanding the Use of In-Cell Solid-State NMR Spectroscopy

Siddarth Narasimhan  1 , Gert E Folkers  1 , Marc Baldus  1
Affiliations
Review

When Small becomes Too Big: Expanding the Use of In-Cell Solid-State NMR Spectroscopy

Siddarth Narasimhan et al. Chempluschem. 2020 Apr.

Abstract

Solution-state NMR spectroscopy has become a powerful tool to study soluble proteins in cells, provided that they tumble sufficiently fast. In addition, cryo-electron tomography (cryo-ET) has recently displayed a tremendous potential to probe structures of large proteins and assemblies in their native cellular environments. However, challenges remain to obtain atomic-level information in native cell settings for proteins that are small, disordered, or are strongly engaged in intermolecular interactions. In this Minireview, we discuss recent progress in using sensitivity enhanced solid-state NMR spectroscopy methods in the context of cellular structural biology.

Keywords: cellular structural biology; dynamic nuclear polarization; in-cell NMR; magic angle spinning; solid-state NMR.

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