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. 2020 Oct;75(10):2668-2670.
doi: 10.1111/all.14325. Epub 2020 May 27.

Highly sensitive ELISA-based assay for quantification of allergen-specific IgE antibody levels

Antonina Karsonova  1 , Ksenja Riabova  1 , Sergio Villazala-Merino  2 , Raffaela Campana  3 , Verena Niederberger  2 , Julia Eckl-Dorna  2 , Renate Fröschl  4 , Thomas Perkmann  4 , Yury V Zhernov  5 , Olga G Elisyutina  5 , Elena S Fedenko  5 , Musa R Khaitov  5 , Daria Fomina  1   6 , Evgeniy Beltiukov  7 , Marianne van Hage  8 , Hans Grönlund  9 , Rudolf Valenta  3   10 , Alexander Karaulov  1 , Mirela Curin  3
Affiliations

Highly sensitive ELISA-based assay for quantification of allergen-specific IgE antibody levels

Antonina Karsonova et al. Allergy. 2020 Oct.
No abstract available

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Conflict of interest statement

RV has received research grants from HVD Life Sciences, Vienna, Austria and Viravaxx, Vienna, Austria, and serves as consultant for Viravaxx. MvH has received lecture fees from Thermo Fisher Scientific and consultancy fees from Biomay AG, Vienna, Austria, and Hycor Biomedical LLC, CA, US, outside of the submitted work. Other authors have no conflict of interest to declare.

Figures

Figure 1
Figure 1
Association of different concentrations of IgEmoAb (x‐axis: ng/mL) with IgE reactivities to Bet v 1 determined as optical density (OD) values (y‐axis) by ELISA (A). Correlation of Bet v 1‐specific IgE levels determined in sera from 19 birch pollen allergic patients by different methods. B, ImmunoCAP (kUA/L) (y‐axis) versus ng/ml determined by ELISA (x‐axis). C, ISU (ISAC standardized unit) values determined by ImmunoCAP ISAC (y‐axis) vs ImmunoCAP (kUA/L) (x‐axis) and D, ISU values determined by ImmunoCAP ISAC (y‐axis) vs ng/mL determined by ELISA (x‐axis). Correlation coefficients (r), regression equations, and statistical significances (P values) are shown in the top upper corner of graph
Figure 2
Figure 2
Correlation of Fel d 1‐specific IgE levels determined in sera from 19 cat allergic patients by ImmunoCAP (kUA/L) (y‐axis) versus ng/ml determined by ELISA (x‐axis) (A). Correlation of Fel d 1‐specific IgE levels determined in lower (y‐axis) and higher (x‐axis) sera dilutions from 19 cat allergic patients from Table S3 (B). Correlation coefficients (r), regression equations, and statistical significances (P values) are shown in the top upper corner of graph
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References

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