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. 1988 Oct;26(10):599-604.
doi: 10.1515/cclm.1988.26.10.599.

Bioluminescent monitoring of ATP release from human red blood cells treated with nonionic detergent

T Köszegi  1 , M Kellermayer, F Kövecs, K Jobst
Affiliations

Bioluminescent monitoring of ATP release from human red blood cells treated with nonionic detergent

T Köszegi et al. J Clin Chem Clin Biochem. 1988 Oct.

Abstract

The adaptation of the firefly luciferin-luciferase system for the detection of ATP in the presence of Triton X 100 or Brij 58 nonionic detergents is described. The method is sensitive, easy to perform and is suitable for the continuous monitoring of the mobilization rate of ATP from Triton X 100- or Brij 58-treated human erythrocytes. A very rapid ATP release was observed after Triton X 100 treatment of the red blood cells while the presence of Brij 58 caused a retarded mobilization of the intracellular ATP. The technique described seems to be applicable to all kinds of cells.

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