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. 2020 Oct;11(10):771-775.
doi: 10.1007/s13238-020-00718-6.

Recapitulation of SARS-CoV-2 infection and cholangiocyte damage with human liver ductal organoids

Bing Zhao #  1 , Chao Ni #  2 , Ran Gao #  3 , Yuyan Wang  4 , Li Yang  2 , Jinsong Wei  2 , Ting Lv  5 , Jianqing Liang  2 , Qisheng Zhang  6 , Wei Xu  4 , Youhua Xie  4 , Xiaoyue Wang  3 , Zhenghong Yuan  4 , Junbo Liang  7 , Rong Zhang  8 , Xinhua Lin  9
Affiliations

Recapitulation of SARS-CoV-2 infection and cholangiocyte damage with human liver ductal organoids

Bing Zhao et al. Protein Cell. 2020 Oct.
No abstract available

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Figures

Figure 1
Figure 1
ACE2+ cholangiocytes are preserved in human liver ductal organoid cultures. (A) Cell-type clusters. t-SNE visualization of the cell populations (color-coded for clusters) from human liver ductal organoids. (B) t-SNE plots indicating the expression of representative marker genes. (C) t-SNE plots indicating the expression of ACE2 and TMPRSS2. (D) Violin plots showing the expression of representative marker genes. (E) Immunofluorescence staining for ACE2 and E-cadherin in human liver ductal organoids. (F) t-SNE visualization of single cells isolated from human and mouse liver ductal organoids; Violin plots showing the expression of EPCAM and ACE2
Figure 2
Figure 2
Recapitulation of SARS-CoV-2 infection in human liver ductal organoids. (A) Immunofluorescence staining for SARS-CoV-2 N protein and E-cadherin in human liver ductal organoids. (B) Two cases of human liver ductal organoids were harvested at indicated time points following SARS-CoV-2 infection to examine the virus load using qRT-PCR. RNP was used as an internal control. (C) Changes in expression of ORF1 in human liver ductal organoids (Org #1 and Org #2) in response to SARS-CoV-2 infection at indicated times. (D) Heatmap showing differentially expressed genes (DEGs) in SARS-CoV-2-infected organoids (Inf) (24 h) versus the mock (Mo). (E) GO analysis of DEGs. Top 10 enriched biological processes were shown. (F) Two cases of human liver ductal organoids after SARS-CoV-2 infection were harvested to examine the expression of indicated genes using qRT-PCR. GAPDH was used as an internal control. Data were presented as mean ± s.d. * indicates P < 0.05; ** indicates P < 0.01; *** indicates P < 0.001 (G) GSEA enrichment analysis of SARS-CoV-2-infected organoids (48 h) versus the mock for cell junction organization signature genes
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References

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