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. 2020 Jul;84(1):e13247.
doi: 10.1111/aji.13247. Epub 2020 May 10.

Amoxicillin-induced gut dysbiosis influences estrous cycle in mice and cytokine expression in the ovary and the caecum

Evandro Neves Silva  1 , Thaís Viana Fialho Martins  1 , Tatimara Maria Miyauchi-Tavares  1 , Bianca Aparecida Expedito Miranda  1 , Gabriela de Assis Dos Santos  1 , Caio Pupin Rosa  2 , Jeferson Antônio Santos  1 , Rômulo Dias Novaes  3 , Leonardo Augusto de Almeida  2 , Patrícia Paiva Corsetti  1
Affiliations

Amoxicillin-induced gut dysbiosis influences estrous cycle in mice and cytokine expression in the ovary and the caecum

Evandro Neves Silva et al. Am J Reprod Immunol. 2020 Jul.

Abstract

Problem: Gut dysbiosis is caused by several factors, including the use of antibiotics. Since intestinal dysbiosis is associated with a wide range of immunopathological and reproductive conditions, the main goal of this study was to evaluate amoxicillin-induced gut dysbiosis and its influence on the oestrous cycle in mice.

Method of study: Mice were treated with amoxicillin or PBS, and faecal microbiota was evaluated by 16S rDNA metagenomic sequencing. The oestrous cycle was evaluated by vaginal cytology, vaginal opening and flow cytometry. After the induction of gut dysbiosis, the ovaries and the caecum were analysed to differential expression of IL-1β and IL-10 genes and histological analysis.

Results: Amoxicillin-treated mice presented differing bacterial groups in the faecal microbiota when compared to the PBS-treated group indicating that amoxicillin treatment-induced gut dysbiosis and they gained weight. The vaginal cytology analysis showed that amoxicillin-induced gut dysbiosis decreased the number of cells but increased the relative number of leucocytes and altered the oestrous cycle. IL-1β was shown to be upregulated in the caecum and in the ovary of the dysbiotic mice. On the other hand, IL-10 expression was shown to be diminished in both organs of the dysbiotic mice. The oocyte area from dysbiotic group presented lower than non-dysbiotic mice with increasing thickness of the pellucid zone. The follicular teak from dysbiotic mice showed lower thickness than non-dysbiotic mice.

Conclusion: The results indicate that amoxicillin induces gut dysbiosis and influences the oestrous cycle and the inflammatory status of the ovary and the caecum.

Keywords: amoxicillin; dysbiosis; immune response; intestinal dysbiosis; oestrous cycle.

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References

REFERENCES

    1. Rosa CP, Brancaglion GA, Miyauchi-tavares TM, Corsetti PP, de Almeida LA. Antibiotic-induced dysbiosis efects on the murine gastrointestinal tract and their systemic repercussions. Life Sci. 2018;207:480-491.
    1. Lynn MA, Tumes DJ, Choo JM, et al. Early-life antibiotic-driven dysbiosis leads to dysregulated vaccine immune responses in mice. Cell Host Microbe. 2018;23:653-660.
    1. Sakai K, Ideta-otsuka M, Saito H, et al. Effects of doxorubicin on sperm DNA methylation in mouse models of testicular toxicity. Biochem Biophys Res Commun. 2018;498:674-679.
    1. Sudman PD, Rutledge JC, Bishop JB, Generoso WM. Bleomycin: female-specific dominant lethal effects in mice. Mutat Res. 1992;296:143-156.
    1. Ianiro G, Tilg H, Gasbarrini A. Antibiotics as deep modulators of gut microbiota: between good and evil. Recent Adv Clin Pract. 2016;65:1906-1915.

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