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. 2020 Jan;49(1):181-185.

Cryopreservation of Echinococcus granulosus Protoscoleces

Alireza Badirzadeh  1 , Saber Raeghi  2 , Vahid Fallah-Omrani  3   4 , Soheila Rouhani  5
Affiliations

Cryopreservation of Echinococcus granulosus Protoscoleces

Alireza Badirzadeh et al. Iran J Public Health. 2020 Jan.

Abstract

Background: The main objective of the current study was to investigate on the cryopreservation of protoscoleces of Echinococcus granulosus, a causative agent of cystic hydatidosis in man.

Methods: This study was conducted on isolated protoscoleces from hydatid cysts infected livers collected from slaughterhouse of Tehran, Iran in 2016. Viability of protoscoleces was evaluated by dye test. Cryopreservation of isolated protoscoleces in the presence of Dimethylsulfoxide (DMSO) and glycerol using a three-step cooling protocol involving an initial period at -20 °C, -80 °C and liquid nitrogen was performed.

Results: The mean viability rate of 10% DMSO and 15% glycerol were 9% and 8% respectively. The protoscoleces of Echinococcus granulosus have been successfully thawed and recovered after 6 months storage in liquid nitrogen.

Conclusion: Cryopreservation method needs to be improved for each species of helminthes and can be useful for other immunological and laboratorial studies.

Keywords: Cryopreservation; Cystic hydatidosis; Dimethylsulfoxide (DMSO); Echinococcus granulosus; Glycerol; Iran.

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Conflict of interest statement

Conflict of interest None declared.

Figures

Fig. 1:
Fig. 1:
Live protoscoleces of E. granulosus which appeared to clear after thawing
Fig. 2:
Fig. 2:
Dead protoscoleces of E. granulosus which appeared to red after thawing
See this image and copyright information in PMC

References

    1. Ananda K, D’Souza PE, Suryanarayana V. (2015). Sero-diagnosis of Echinococcus granulosus infection in dogs using faecal supernatant antigen. J Parasit Dis, 39(3):461–4. - PMC - PubMed
    1. McManus DP, Zhang W, Li J, Bartley PB. (2003). Echinococcosis. Lancet, 362(9392):1295–304. - PubMed
    1. Juyi L, Yan J, Xiufang W, et al. (2013). Analysis of the chemical components of hydatid fluid from Echinococcus granulosus. Rev Soc Bras Med Trop, 46(5):605–10. - PubMed
    1. Eckert J. (1988). Cryopreservation of parasites. Experientia, 44(10):873–7. - PubMed
    1. Leef JL, Hollingdale MR, Beaudoin RL. (1981). Principles of cryopreservation of protozoan parasites and erythrocytes. Wld Hlth Org Document, Mal 81: 940.

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