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. 2020 Mar 20:30:105416.
doi: 10.1016/j.dib.2020.105416. eCollection 2020 Jun.

Dataset for the combined transcriptome assembly of M. oleifera and functional annotation

K Mohamed Shafi  1   2 , Adwait G Joshi  1 , Iyer Meenakshi  1 , Shaik Naseer Pasha  1 , K Harini  1 , Jarjapu Mahita  1 , Radha Sivarajan Sajeevan  1 , Snehal D Karpe  1 , Pritha Ghosh  1 , Sathyanarayanan Nitish  1   2 , A Gandhimathi  1 , Oommen K Mathew  1 , Subramanian Hari Prasanna  1 , Manoharan Malini  1 , Eshita Mutt  1 , Mahantesha Naika  1 , Nithin Ravooru  1 , Rajas M Rao  1 , Prashant N Shingate  1 , Anshul Sukhwal  1 , Margaret S Sunitha  1 , Atul K Upadhyay  1   3 , Rithvik S Vinekar  1 , Ramanathan Sowdhamini  1
Affiliations

Dataset for the combined transcriptome assembly of M. oleifera and functional annotation

K Mohamed Shafi et al. Data Brief. .

Abstract

In this paper, we present the data acquired during transcriptome analysis of the plant Moringa oleifera [1] from five different tissues (root, stem, leaf, flower and seed) by RNA sequencing. A total of 271 million reads were assembled with an N50 of 2094 bp. The combined transcriptome was assessed for transcript abundance across five tissues. The protein coding genes identified from the transcripts were annotated and used for orthology analysis. Further, enzymes involved in the biosynthesis of select medicinally important secondary metabolites, vitamins and ion transporters were identified and their expression levels across tissues were examined. The data generated by RNA sequencing has been deposited to NCBI public repository under the accession number PRJNA394193 (https://www.ncbi.nlm.nih.gov/bioproject/PRJNA394193).

Keywords: Annotation; Enrichment analysis; Gene expression; Metabolic pathway; Orthology; Transcriptome.

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Conflict of interest statement

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Figures

Fig. 1
Fig. 1
The orthogroup distribution of M. oleifera proteins compared across 37 plant species. The orthogroup distribution contains 38 classes from 1 to 38 – each representing the number of orthogroups from the species which are shared with zero to thirty seven of the other species in the analysis, respectively (e.g. ‘1′ - first stack of bar plot contains the unique proteins in the proteome).
Fig. 2
Fig. 2
The GO terms enriched in top 100 abundant transcripts from all tissues for (A) biological process, (B) molecular function and (C) cellular component. The tree map was generated using REVIGO visualization tool. Each rectangle represent a single cluster of GO terms, visualized using different colors. Size of the each cluster indicates the p-value (less than 0.05).
Fig. 3
Fig. 3
Schematic representation of tissue-specific abundance of transcripts coding for enzymes involved in secondary metabolite and vitamin biosynthesis and ion transporters. The minerals enriched in different tissues have also been indicated.
See this image and copyright information in PMC

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