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. 2020 Jun;17(6):647-649.
doi: 10.1038/s41423-020-0426-7. Epub 2020 Apr 20.

Human monoclonal antibodies block the binding of SARS-CoV-2 spike protein to angiotensin converting enzyme 2 receptor

Xiangyu Chen #  1 , Ren Li #  2   3 , Zhiwei Pan #  1 , Chunfang Qian #  4 , Yang Yang  1 , Renrong You  5 , Jing Zhao  6 , Pinghuang Liu  5 , Leiqiong Gao  1 , Zhirong Li  1 , Qizhao Huang  7 , Lifan Xu  1 , Jianfang Tang  1 , Qin Tian  1 , Wei Yao  1 , Li Hu  1 , Xiaofeng Yan  4 , Xinyuan Zhou  1 , Yuzhang Wu  1 , Kai Deng  8 , Zheng Zhang  9 , Zhaohui Qian  10 , Yaokai Chen  11 , Lilin Ye  12
Affiliations

Human monoclonal antibodies block the binding of SARS-CoV-2 spike protein to angiotensin converting enzyme 2 receptor

Xiangyu Chen et al. Cell Mol Immunol. 2020 Jun.
No abstract available

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Conflict of interest statement

The authors declare no competing interests.

Figures

Fig. 1
Fig. 1
Human monoclonal antibodies block the SARS-CoV-2 RBD protein-hACE2 protein interaction a ELISA binding assay of COVID-19 patient sera to ELISA plate coating of SARS-CoV-2 S1 protein. b ELISA binding assay of COVID-19 patient sera to ELISA plate coating of SARS-CoV-2 RBD protein. c COVID-19 patient serum-mediated inhibition of the SARS-CoV-2 S1 protein binding to hACE2 protein by ELISA. d An overall strategy of anti-SARS-CoV-2 RBD mAbs. e Flow cytometry analysis of SARS-CoV-2 RBD-specific IgG+ B cells in PBMCs of healthy donor and patient XFQ. f Specificity of mAbs (311mab–31B5, −32D4 and −31B9 clones) to SARS-CoV-2 RBD protein by ELISA. g ELISA analysis of SARS-CoV-2 RBD-hACE2 interaction inhibited by 311mab–31B5, −32D4, and −31B9 mAbs. h Flow cytometry analysis of SARS-CoV-2 RBD-hACE2 interaction inhibited by 311mab–31B5, −32D4, and −31B9 mAbs. The numbers adjacent to the outlined areas indicate the percentages of anti-mouse IgG+ hACE2-plasmid transiently transfected 293T cells, which are summarized in i (left panel). i (right panel) Mean fluorescence intensity (MFI) of Alexa Fluor 647 anti-mouse IgG in anti-mouse IgG+ hACE2-plasmid transiently transfected 293T cells. j Antibody-mediated blocking of luciferase-encoding SARS-Cov-2 typed pseudovirus into hACE2/293T cells. NC, negative control. HD, healthy donor. The data are representative of two independent experiments with three replicates per group (f, g, i, j; error bars in f, g, i, j indicate the SD)
See this image and copyright information in PMC

References

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