Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2020 Apr 17;9(4):192.
doi: 10.3390/antibiotics9040192.

Genomic Characterization of Escherichia coli Isolates Belonging to a New Hybrid aEPEC/ExPEC Pathotype O153:H10-A-ST10 eae-beta1 Occurred in Meat, Poultry, Wildlife and Human Diarrheagenic Samples

Dafne Díaz-Jiménez  1   2 , Isidro García-Meniño  1   2 , Alexandra Herrera  1 , Vanesa García  1   2   3 , Ana María López-Beceiro  4 , María Pilar Alonso  5 , Jorge Blanco  1   2 , Azucena Mora  1   2
Affiliations

Genomic Characterization of Escherichia coli Isolates Belonging to a New Hybrid aEPEC/ExPEC Pathotype O153:H10-A-ST10 eae-beta1 Occurred in Meat, Poultry, Wildlife and Human Diarrheagenic Samples

Dafne Díaz-Jiménez et al. Antibiotics (Basel). .

Abstract

Different surveillance studies (2005-2015) in northwest Spain revealed the presence of eae-positive isolates of Escherichia coli O153:H10 in meat for human consumption, poultry farm, wildlife and human diarrheagenic samples. The aim of this study was to explore the genetic and genomic relatedness between human and animal/meat isolates, as well as the mechanism of its persistence. We also wanted to know whether it was a geographically restricted lineage, or whether it was also reported elsewhere. Conventional typing showed that 32 isolates were O153:H10-A-ST10 fimH54, fimAvMT78, traT and eae-beta1. Amongst these, 21 were CTX-M-32 or SHV-12 producers. The PFGE XbaI-macrorestriction comparison showed high similarity (>85%). The plasmidome analysis revealed a stable combination of IncF (F2:A-:B-), IncI1 (STunknown) and IncX1 plasmid types, together with non-conjugative Col-like plasmids. The core genome investigation based on the cgMLST scheme from EnteroBase proved close relatedness between isolates of human and animal origin. Our results demonstrate that a hybrid MDR aEPEC/ExPEC of the clonal group O153:H10-A-ST10 (CH11-54) is circulating in our region within different hosts, including wildlife. It seems implicated in human diarrhea via meat transmission, and in the spreading of ESBL genes (mainly of CTX-M-32 type). We found genomic evidence of a related hybrid aEPEC/ExPEC in at least one other country.

Keywords: EPEC; ESBL; EnteroBase; Escherichia coli; ExPEC; MDR; O153; ST10; hybrid pathotype.

PubMed Disclaimer

Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Pulsed field gel electrophoresis (PFGE) of XbaI-digested DNA from 20 ESBL-producing aEPEC isolates of the clonal group O153:H10-A-ST10 (one autodigested). On the right of the dendrogram: Isolate designation, O:H serotype, ST, ESBL type, phylogroup, year of isolation, geographic origin, source and virulence-gene profile.
Figure 2
Figure 2
GrapeTree inferred using the MSTree V2 algorithm based on the cgMLST V1 + Hierarchical Clustering (HierCC) V1 scheme from EnteroBase.
Figure 3
Figure 3
Dendrogram based on the SNPs of the core genomic regions present in 90% of the compared genomes and using LREC-113 as reference, built in EnteroBase and modified with FigTree v1.4.3.
See this image and copyright information in PMC

References

    1. Kaper J.B., Nataro J.P., Mobley H.L. Pathogenic Escherichia coli. Nat. Rev. Microbiol. 2004;2:123–140. doi: 10.1038/nrmicro818. - DOI - PubMed
    1. Riley L.W. Pandemic lineages of extraintestinal pathogenic Escherichia coli. Clin. Microbiol. Infect. 2014;20:380–390. doi: 10.1111/1469-0691.12646. - DOI - PubMed
    1. Caron E., Crepin V.F., Simpson N., Knutton S., Garmendia J., Frankel G. Subversion of actin dynamics by EPEC and EHEC. Curr. Opin. Microbiol. 2006;9:40–45. doi: 10.1016/j.mib.2005.12.008. - DOI - PubMed
    1. Mora A., Blanco M., Yamamoto D., Dahbi G., Blanco J.E., Lopez C., Alonso M.P., Vieira M.A., Hernandes R.T., Abe C.M., et al. HeLa-cell adherence patterns and actin aggregation of enteropathogenic Escherichia coli (EPEC) and Shiga-toxin-producing E. coli (STEC) strains carrying different eae and tir alleles. Int. Microbiol. 2009;12:243–251. - PubMed
    1. Blanco M., Blanco J.E., Mora A., Dahbi G., Alonso M.P., Gonzalez E.A., Bernardez M.I., Blanco J. Serotypes, virulence genes, and intimin types of Shiga toxin (verotoxin)-producing Escherichia coli isolates from cattle in Spain and identification of a new intimin variant gene (eae-xi) J. Clin. Microbiol. 2004;42:645–651. doi: 10.1128/JCM.42.2.645-651.2004. - DOI - PMC - PubMed

LinkOut - more resources