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. 2020 Feb 28;9(2):1003-1008.
doi: 10.4103/jfmpc.jfmpc_863_19. eCollection 2020 Feb.

Qualitative and quantitative analysis of bacterial aerosols in dental clinical settings: Risk exposure towards dentist, auxiliary staff, and patients

Manish Jain  1 , Aditi Mathur  2 , Anmol Mathur  3 , Pravin U Mukhi  4 , Mahesh Ahire  5 , Chadrashekhar Pingal  6
Affiliations

Qualitative and quantitative analysis of bacterial aerosols in dental clinical settings: Risk exposure towards dentist, auxiliary staff, and patients

Manish Jain et al. J Family Med Prim Care. .

Abstract

Introduction: Cross-infection or contamination are the major threats related to any medical profession. Microorganisms present in the dental clinic can cause cross-infection to the dentist, auxiliary staff and even towards the patients.

Aims: The study was conducted to assess the level of atmospheric microbial contamination and composition of aerosols before, during and after dental treatment procedures in four clinical settings.

Methods and materials: The present study was conducted over a two-week period in a private dental college setting. An equal number of culture medium plates (blood agar) were placed 30 min prior to the initiation of work sessions in the selected area and 1 h after the working session began and after 2 h of cessation of the working period. After the collection of samples, the culture medium plates were incubated aerobically at 37°C in an incubator for 48 h. The number of colonies was expressed as colonies per media plate. After counting the colonies bacterial cell morphology was determined by a microscopic examination using a Reichert-Jung Series 150 light microscope.

Statistical analysis: Statistical analysis such as ANOVA test for mean values and post hock was done using statistical package for social sciences (SPSS).

Results: It shows that colony count increased after the working session and which reduced by itself once the working session was concluded which was significant (P < 0.001). The highest increase in the mean colony count was found in the department of periodontology during the treatment sessions. In the blood agar plates, the S. epidermidis was found maximum 62%, micrococcus was 22%, diphtheroid was 10%, fungi 4% and the least S. aureus 2%.

Conclusion: This study demonstrates that aerosols increase during and after work sessions and, therefore, increases the chance for infectious agent transmission in clinical settings.

Keywords: Bacterial aerosols; clinical dentistry; cross-infection; microbial contamination.

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Conflict of interest statement

There are no conflicts of interest.

Figures

Figure 1
Figure 1
Comparison of mean colony count for the departments before treatment P-value>0.889 (NS)
Figure 2
Figure 2
Comparison of mean colony count for the departments during the treatment P-value < 0.001(S)
Figure 3
Figure 3
Comparison of mean colony count for the departments after the treatment P-value < 0.05 (S)
Figure 4
Figure 4
Comparison of mean colony count for the sterilization room of the oral surgery department (before and after the treatment) P-value< 0.01 (S)
Figure 5
Figure 5
Percentage of microorganism colony composition
See this image and copyright information in PMC

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