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Comparative Study
. 2020 Apr 1;21(4):1039-1044.
doi: 10.31557/APJCP.2020.21.4.1039.

A Comparison between Three Different Automated Total 25-hydroxyvitamin D Immunoassay Methods and Ultra Performance Liquid Chromatography

Hala Reda  1 , Sonya Soliman  1   2 , Hany Girguis  2 , Mohamed Nagy  3 , Yousra Mahmoud  3 , Nouran Yasser  4
Affiliations
Comparative Study

A Comparison between Three Different Automated Total 25-hydroxyvitamin D Immunoassay Methods and Ultra Performance Liquid Chromatography

Hala Reda et al. Asian Pac J Cancer Prev. .

Abstract

Objective: Vitamin D is a fat soluble vitamin responsible for calcium metabolism and more recently discovered effects. This led to an increase in requests for vitamin D test by clinicians. New automated assays have been introduced for 25-hydroxyvitamin D measurement.

Methods: Results from these new method have to be related to a Standard method to obtain best results for practical usage. In our study, one hundred venous blood samples were analyzed for 25-OH vitamin D on three immunological methods in our lab and correlated with ultra-performance liquid chromatography (UPLC) method as a reference method.

Results: Statistically analysis of results obtained for correlations between the 3 methods against the reference UPLC was done by Spearman's Correlation. It showed positive correlation in all methods with significant p value < 0.001. Differences and biases between methods were evaluated using a Bland-Altman plot and Cohen's Kappa agreement. Best agreement was found in Cobas 6000 followed by the Access2 then comes Architect. Conclusions: All immunoassays can be used in routine 25(OH) D measurements, still some methods are better than others. A clinical laboratory must at least be aware of its method to avoid misinterpretation of results.

Keywords: 25-Hydroxy vitamin D; Vitamin D; Vitamin D method comparison; Vitamin D standardization; vitamin D chromatography.

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Figures

Figure 1
Figure 1
Comparison of the Abbott Architect with UPLC Method using Bland-Altman Analysis
Figure 2
Figure 2
Comparison of the Cobas e601 with UPLC Using Bland-Altman Analysis
Figure 3
Figure 3
Comparison of the Access2 with UPLC using Bland-Altman Analysis
See this image and copyright information in PMC

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