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. 2020 May;27(5):1390-1395.
doi: 10.1016/j.sjbs.2019.11.038. Epub 2019 Dec 2.

Indigofera oblongifolia as a fight against hepatic injury caused by murine trypanosomiasis

Mohamed A Dkhil  1   2 , Rewaida Abdel-Gaber  1   3 , Mona F Khalil  2 , Taghreed A Hafiz  4 , Murad A Mubaraki  4 , Esam M Al-Shaebi  1 , Saleh Al-Quraishy  1
Affiliations

Indigofera oblongifolia as a fight against hepatic injury caused by murine trypanosomiasis

Mohamed A Dkhil et al. Saudi J Biol Sci. 2020 May.

Abstract

Trypanosoma evansi is a hazardous pathogenic parasite infecting a broad variety of livestock and affects wildlife worldwide. Trypanosoma evansi has gained resistance to most drugs used; therefore, it requires alternative medicines. The objective of this research was to investigate the impact of Indigofera oblongifolia leaf extract (IE) on T. evansi-induced hepatic injury. Mice were once infected with 1000 T. evansi. The treated group was gavaged with 100 mg/Kg IE after infection. Histological and biochemical changes in mice hepatic tissue were studied. Also, the oxidative damage in the liver was evaluated through determining the level of glutathione (GSH), Malondialdehyde (MDA), nitric oxide (NO) and catalase (CAT) markers. IE was able to suppress the induced parasitemia due to infection. Also, IE improved the histological liver architecture. Furthermore, the liver enzymes, alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) activity were improved after IE mice were treated. IE protects against hepatic damage caused by trypanosomiasis in mice. Further studies are needed to isolate the active compounds in IE and to monitor these compunds' ameliorative function.

Keywords: Indigofera oblongifolia; Liver damage; Mouse; Oxidative status; Trypanosoma.

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Figures

Fig. 1
Fig. 1
Infrared spectroscopy of I. oblongifolia leaf extracts.
Fig. 2
Fig. 2
Blood smear of T. evansi-infected (A) and infected- I. oblongifolia treated mice (B). Smears were stained with Giemsa. Bar = 50 µm.
Fig. 3
Fig. 3
Effect of I. oblongifolia on liver histology of infected with T. evansi. (A,B), control mice with normal histology. (C,D), Infected liver with inflammation, hemorrhage, hyperplasia of Kupffer cells and many trypanosomes in the central vein. (E,F), Infected-treated liver with improved histological structure.
Fig. 4
Fig. 4
Effect of I. oblongifolia on liver enzymes, AST, ALT and ALP in the blood plasma of T. evansi infected mice.
See this image and copyright information in PMC

References

    1. Aebi H. Catalase in vitro. Methods Enzymol. 1984;105:121–126. - PubMed
    1. Al-Otaibi M.S.A., Al-Quraishy S., Al-Malki E., Abdel-Baki A.S. Therapeutic potential of the methanolic extract of Lepidium sativum seeds on mice infected with Trypanosoma evansi. Saudi J. Biol. Sci. 2018;26:1473–1477. doi: 10.1016/j.sjbs.2018.08.031. - DOI - PMC - PubMed
    1. Anosa V.O. Haematological and biochemical changes in human and animal trypanosomiasis. Part II. Rev. Elev. Med. Vet. Pays. Trop. 1988;41:151–164. - PubMed
    1. Bal M.S., Singla L.D., Kumar H., Vasudev A., Gupta K., Juyal P.D. Pathological studies on experimental Trypanosoma evansi infection in Swiss albino mice. J. Parasit. Dis. 2012;36:260–264. - PMC - PubMed
    1. Biswas D., Choudhury A., Misra K.K. Histopathology of Trypanosoma (Trypanozoon) evansi infection in bandicoot rat. I. Visceral organs. Exp. Parasitol. 2001;99:148–159. - PubMed

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