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Comparative Study
. 2020 Dec;58(1):367-373.
doi: 10.1080/13880209.2020.1755318.

Comparison of the short-chain fatty acids in normal rat faeces after the treatment of Euphorbia kansui, a traditional Chinese medicine for edoema

Dongjing Jiang  1   2 , Sijia Guo  2 , An Kang  2 , Yonghui Ju  2 , Jingxian Li  2 , Sheng Yu  2 , Beihua Bao  2 , Yudan Cao  2 , Yuping Tang  3 , Li Zhang  2 , Weifeng Yao  2
Affiliations
Comparative Study

Comparison of the short-chain fatty acids in normal rat faeces after the treatment of Euphorbia kansui, a traditional Chinese medicine for edoema

Dongjing Jiang et al. Pharm Biol. 2020 Dec.

Abstract

Context: As a toxic traditional Chinese medicine for edoema, Euphorbia kansui S.L. Liou ex S.B. Ho (Euphorbiaceae) (EK) stir-fried with vinegar for detoxification was associated with alterations of gut microbiota. However, the evidence of correlation between short-chain fatty acids (SCFAs) and toxicity of EK has not been confirmed.Objective: In order to study the biological basis of detoxification of EK stir-fried with vinegar (VEK), a rapid, sensitive and validated GC-MS method was developed to determine SCFAs in normal rat faeces after given EK and VEK.Materials and methods: Sprague Dawley rats were orally administered 0.5% CMC-Na (control group), EK (EK-treated group) and VEK powder (VEK-treated group) at 680 mg/kg for six consecutive days (eight rats each group). Fresh faeces samples were promptly collected, derivatized and then analyzed by GC-MS.Results: The ranges of LOD and LOQ were within 0.13-1.79 and 0.45-5.95 μg/mL, respectively. The RSD values of intra-day and inter-day precisions were less than 15%. Four SCFAs were generally stable under four storage conditions. The extraction recoveries were ranged from 53.5% to 97.3% with RSD values lower than 15%. The concentrations of four SCFAs in EK and VEK were decreased significantly compared with those not administered (EK-treated, p < 0.01; VEK-treated, p < 0.05 and p < 0.01). After being stir-fried with vinegar, the concentrations were all increased (p < 0.05 and p < 0.01).Discussion and conclusions: The negative correlation between SCFAs and toxicity of EK may provide evidence for biological mechanism and toxic Chinese medicine.

Keywords: Euphorbiaceae; biological sample; stir-fried with vinegar; toxicity; volatile fatty acids.

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Conflict of interest statement

There are no conflicts of interest regarding the publication of this article.

Figures

Figure 1.
Figure 1.
Typical GC-MS chromatography of faeces spiked with standard mixture solutions (A) and faecal sample (B) Peak identification: 1, acetic acid; 2, propionic acid; 3, butyric acid; 4, valeric acid; IS.
See this image and copyright information in PMC

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