Advances of droplet-based microfluidics in drug discovery

Abstract

Introduction: Drug discovery through a large number of candidates with nearly unlimited possibilities is laborious, time-consuming, and incurs massive costs. It thus calls for fast and robust methods that allow for handling small amounts of reagents, automation, and high-throughput analysis. In this regard, droplet-based microfluidic platforms provide several unique advantages. Highly monodispersed droplets can be generated in a high frequency and serve as microcontainers with just a few femtoliter to nanoliter volume. Furthermore, each of them can be manipulated to initiate specific reactions in parallel. Consequently, droplet microfluidics is emerging as a useful tool for drug discovery and development.

Areas covered: The authors provide a brief overview of the latest developments of droplet-based microfluidic techniques for drug discovery and emphasize their applications at different stages, covering target selection, drug candidate identification, and preclinical research.

Expert opinion: Droplet-based microfluidics holds great potential in drug discovery due to its capability of reaction miniaturization and high-throughput analysis. However, its commercial applications are still at an early stage as the experiments are mostly implemented utilizing custom-built instruments in laboratory environments. Thus, joint efforts from scientists and engineers with multidisciplinary backgrounds are required to optimize the standardization and stability of droplet-based microfluidic platforms.

Keywords: Droplet-based microfluidics; drug discovery; lead identification; preclinical studies; target selection.