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. 2020;151(2 & 3):244-250.
doi: 10.4103/ijmr.IJMR_1029_20.

First isolation of SARS-CoV-2 from clinical samples in India

Prasad Sarkale  1 , Savita Patil  1 , Pragya D Yadav  1 , Dimpal A Nyayanit  1 , Gajanan Sapkal  2 , Shrikant Baradkar  1 , Rajen Lakra  1 , Anita Shete-Aich  1 , Sharda Prasad  3 , Atanu Basu  3 , Lalit Dar  4 , Veena Vipat  5 , Sidhartha Giri  6 , Varsha Potdar  5 , Manohar Lal Choudhary  5 , Ira Praharaj  6 , Amita Jain  7 , Bharati Malhotra  8 , Pranita Gawande  1 , Kaumudi Kalele  1 , Nivedita Gupta  6 , Sarah S Cherian  9 , Priya Abraham  10
Affiliations

First isolation of SARS-CoV-2 from clinical samples in India

Prasad Sarkale et al. Indian J Med Res. 2020.
No abstract available

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Conflict of interest statement

None

Figures

Fig. 1
Fig. 1
Immunofluorescence images (red panel) showing uninfected Vero CCL-81 cells probed by positive patient serum samples after post infection day of 13th (left), 11th (middle) and seventh (right) and with SARS-CoV-2 strain NIV-2020-770 infected Vero CCL-81 cells probed by positive patients serum (green panel) showing the reactivity of virus and antibody.
Fig. 2
Fig. 2
Cytopathic effect of the SARS-CoV-2 isolate (NIV-2020-770) demonstrated in Vero CCL-81 cells on different post-infection days (PID).
Fig. 3
Fig. 3
Transmission electron microscopy imaging of SARS-CoV-2. A negative-stained SARS-CoV-2 viral particle, demonstrating spike morphology of glycoprotein along with peplomeric projections, a feature typical to the family Coronaviridae, is seen.
Fig. 4
Fig. 4
Neighbour-joining tree of SARS-CoV-2. The phylogenetic tree is generated using the best substitution model. A bootstrap of 1000 replicates was used to assess the statistical robustness of the tree. Same colours are used for sequences derived from a clinical sample and the respective isolate. Clinical samples are labelled with initials as nCoV while the isolates are labelled with initials as NIV. The clades are represented by different colours in the core region (S - yellow, V - pink, G - blue and unclassified - not coloured).
See this image and copyright information in PMC

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