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. 2020 Apr 28;64(2):3117.
doi: 10.4081/ejh.2020.3117.

The substance P and neurokinin-1 receptor system in human thyroid cancer: an immunohistochemical study

Inmaculada Isorna  1 , Francisco Esteban  2 , Juan Solanellas  3 , Rafael Coveñas  4 , Miguel Muñoz  5
Affiliations

The substance P and neurokinin-1 receptor system in human thyroid cancer: an immunohistochemical study

Inmaculada Isorna et al. Eur J Histochem. .

Abstract

To develop a new therapeutic strategy against thyroid cancer (TC), the expression of both substance P (SP) and neurokinin-1 receptor (NK-1R) must be demonstrated in TC cells. This study aims to examine by immunohistochemistry, the localization of SP and the NK-1R in human TC samples (papillary, follicular, medullary, anaplastic), in metastasis and in healthy thyroid samples. SP and the NK-1R were expressed in all normal and TC samples. In healthy glands, SP was located in follicular cells (nucleus) and colloid and NK-1R in follicular cells (cytoplasm) and stroma. In TC samples, SP was visualized in follicular cells (nucleus and cytoplasm), stroma and colloid and NK-1R in follicular cells (cytoplasm), stroma and colloid. A semiquantitative scoring system (Allred Unit Scoring System) was applied. The expression (Allred total score) of SP and NK-1R was weaker in normal thyroid glands than in TC. In comparison with TC samples, a lower intensity/proportion of SP (nucleus and cytoplasm of follicular cells; stroma) was observed in normal samples. By contrast, in the colloid of TC samples the presence of SP was lower than in normal samples. In comparison with TC samples, the presence of the NK-1R in the cytoplasm of follicular cells and colloid was lower in normal thyroid samples, whereas the expression of this receptor in the stroma was higher. The results reported in this study suggest that the NK-1R could be a new target for the treatment of TC and use of the NK-1R antagonists could serve as a new anti-TC therapeutic strategy.

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Conflict of interest statement

Conflict of interest: The authors declare that they have no competing interests, and all authors confirm accuracy. USPTO Application no. 20090012086 “Use of non-peptidic NK-1 receptor antagonists for the production of apoptosis in tumor cells” (Miguel Muñoz).

Figures

Figure 1.
Figure 1.
Thyroid samples: SP expression showing an intense nuclear pattern (arrows). A) Healthy thyroid. B) Negative control. C) PTC. D) FTC. E) Hürthle cell variant TC carcinoma. F) MTC. G) MeTC. H) ATC.
Figure 2.
Figure 2.
Thyroid samples: NK-1R expression showing a predominantly cytoplasmic pattern (arrows). A) Healthy thyroid. B) Negative control. C) PTC. D) FTC. E) Hürthle cell variant TC carcinoma. F) MTC. G) MeTC. H) ATC.
Figure 3.
Figure 3.
SP expression in large blood vessels (FTC sample). Immunoreactive nucleus in the endothelial cell (black arrow). No immunoreactivity in tunica media/intima (blue arrow).
See this image and copyright information in PMC

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