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. 2020 Nov;44(11):E482-E493.
doi: 10.1111/aor.13718. Epub 2020 Jul 1.

In vivo evaluation of Vivere bovine pericardium valvular bioprosthesis with a new anti-calcifying treatment

Maria C V B Braile-Sternieri  1   2 , Gilberto Goissis  1 , Aparecida de F Giglioti  1 , Vladimir D A Ramirez  1 , Nathalia P Pereira  1 , Adriano de Vasconcellos  1 , Glaucia G Basso-Frazzato  1 , Domingo Marcolino Braile  1   3   4
Affiliations

In vivo evaluation of Vivere bovine pericardium valvular bioprosthesis with a new anti-calcifying treatment

Maria C V B Braile-Sternieri et al. Artif Organs. 2020 Nov.

Abstract

The objective of this study was to evaluate the effect of chemical treatment with glutamic acid to avoid calcification of biological cardiac valves. The bovine pericardium (BP) tissues were fixed with 0.5% glutaraldehyde (BP/GA), followed by treatment with glutamic acid (BP/GA + Glu) for neutralization of the free aldehyde groups. Microscopic analysis showed that the wavy structure of collagen fibrils was preserved, but changes in elastin's integrity occurred. However, the treatment did not promote undesirable changes in the thermal and mechanical properties of the modified BPs. These samples were systematically studied in rat subcutaneous tissue: control (BP/GA) and anticalcificant (BP/GA + Glu). After 60 days, both groups induced similar inflammatory reactions. In terms of calcification, BP/GA + Glu remained more stable with a lower index (3.1 ± 0.2 μg Ca2+ /mg dry tissue), whereas for BP/GA it was 5.7 ± 1.3 μg Ca2+ /mg dry tissue. Bioprostheses made from BP/GA + Glu were implanted in the pulmonary position in sheep, and in vivo echocardiographic analyses revealed maintenance of valvar function after 180 days, with low gradients and minimal valve insufficiency. The explanted tissues of the BP/GA + Glu group had a lower average calcium content 3.8 ± 3.0 μg Ca2+ /mg dry tissue. The results indicated high anticalcification efficiency of BP/GA + Glu in both subcutaneous implant in rats and in the experimental sheep model, which is an advantage that should encourage the industrial application of these materials for the manufacture of bioprostheses.

Keywords: anticalcificant treatment; bovine pericardium; calcification; crosslinking; glutamic acid; glutaraldehyde.

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References

REFERENCES

    1. Tam H, Zhang W, Infante D, Parchment N, Vyavahare N. Fixation of bovine pericardium-based tissue biomaterial with irreversible chemistry improves biochemical and biomechanical properties. J Cardiovasc Transl Res. 2017;10(2):194-205.
    1. Lee S, Levy RJ, Christian AJ, Hazen SL, Frick NE, Lai EK, et al. Calcification and oxidative modifications are associated with progressive bioprosthetic heart valve dysfunction. J Am Heart Assoc. 2017;6(5):e005648.
    1. Cohn LH, Collins JJ, Couper GS, Aranki SF. Twenty-year follow-up of the Hancock modified orifice porcine aortic valve. Ann Thorac Surg. 1998;66(6):S30-4.
    1. Alvarez JR, Sierra J, Vega M, Adrio B, Martinez-Comendador J, Gude F, et al. Early calcification of the aortic Mitroflow pericardial bioprosthesis in the elderly. Interact Cardiovasc Thorac Surg. 2009;9(5):842-6.
    1. Johnston DR, Soltesz EG, Vakil N, Rajeswaran J, Roselli EE, Sabik JF, et al. Long-term durability of bioprosthetic aortic valves: implications from 12,569 implants. Ann Thorac Surg. 2015;99(4):1239-47.

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