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. 2020 May 1;12(5):1285.
doi: 10.3390/nu12051285.

Blueberry Prevents the Bladder Dysfunction in Bladder Outlet Obstruction Rats by Attenuating Oxidative Stress and Suppressing Bladder Remodeling

Nozomu Miyazaki  1 , Ryota Katsura  1 , Koki Hamada  1 , Tatsuo Suzutani  1
Affiliations

Blueberry Prevents the Bladder Dysfunction in Bladder Outlet Obstruction Rats by Attenuating Oxidative Stress and Suppressing Bladder Remodeling

Nozomu Miyazaki et al. Nutrients. .

Abstract

Various berries demonstrate antioxidant activity, and this effect is expected to prevent chronic diseases. We examined whether a diet containing blueberry powder could prevent the development of bladder dysfunction secondary to bladder outlet obstruction (BOO). Eighteen 8-week-old male Sprague-Dawley rats were randomly divided into three groups: Sham (sham operated + normal diet), N-BOO (BOO operated + normal diet) and B-BOO (BOO operated + blueberry diet). Four weeks after BOO surgery, the N-BOO group developed bladder dysfunction with detrusor overactivity. The B-BOO group showed significantly improved micturition volume and micturition interval. The urinary levels of 8-hydroxy-2'-deoxyguanosine (8-OHdG) and malondialdehyde (MDA) were measured as oxidative stress markers. In the N-BOO group, 8-OHdG increased 1.6-fold and MDA increased 1.3-fold at 4 weeks after surgery, whereas the increase in 8-OHdG was significantly reduced by 1.1-fold, despite a similar increase in MDA, in the B-BOO group. Bladder remodeling was confirmed due to bladder hypertrophy, fibrosis and increased connexin43 expression in the N-BOO group, but these histological changes were reduced in the B-BOO group. The intake of blueberries prevented the development of bladder dysfunction secondary to BOO. This effect seems to be related to antioxidation and the inhibition of bladder remodeling.

Keywords: Bladder Outlet Obstruction; Overactive Bladder Symptoms; blueberry; functional food; rat model.

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Conflict of interest statement

The authors declare no conflicts of interest.

Figures

Figure 1
Figure 1
Representative cystometrogram recordings for the Sham, N-BOO and B-BOO groups.
Figure 2
Figure 2
Anti-oxidative effect of blueberries on urinary 8-OHdG (A) and malondialdehyde (MDA) (B). A single asterisk indicates p <0.05. Double asterisks indicate p <0.01.
Figure 3
Figure 3
Histological analysis of the bladder tissue from Sham, N-BOO and B-BOO group rats. (A) Hematoxylin–Eosin staining (HE) is shown in the upper row and Masson trichrome stain (MT) in the lower row. (B) Mean ± SE of 6 determinations each of the percentage of collagen in muscle layer of Sham, N-BOO and B-BOO group rat bladders. A single asterisk indicates p <0.05. Double asterisks indicate p <0.01.
Figure 4
Figure 4
Representative Western blots of (A) Cx43 expression, and (B) connective tissue growth factor (CTGF) expression in the bladder (upper). Relative expression levels of (A) Cx43/beta-actin; (B) CTGF/beta-actin in the bladder (lower). A single asterisk indicates p <0.05.
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