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. 2020 May;17(5):509-513.
doi: 10.1038/s41592-020-0817-9. Epub 2020 May 4.

Three-photon head-mounted microscope for imaging deep cortical layers in freely moving rats

Alexandr Klioutchnikov #  1 , Damian J Wallace #  1 , Michael H Frosz  2 , Richard Zeltner  2 , Juergen Sawinski  1 , Verena Pawlak  1 , Kay-Michael Voit  1 , Philip St J Russell  2 , Jason N D Kerr  3
Affiliations

Three-photon head-mounted microscope for imaging deep cortical layers in freely moving rats

Alexandr Klioutchnikov et al. Nat Methods. 2020 May.

Abstract

We designed a head-mounted three-photon microscope for imaging deep cortical layer neuronal activity in a freely moving rat. Delivery of high-energy excitation pulses at 1,320 nm required both a hollow-core fiber whose transmission properties did not change with fiber movement and dispersion compensation. These developments enabled imaging at >1.1 mm below the cortical surface and stable imaging of layer 5 neuronal activity for >1 h in freely moving rats performing a range of behaviors.

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