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. 1988 Oct;214(2):333-42.
doi: 10.1007/BF00337732.

5S rRNA genes in Pisum: sequence, long range and chromosomal organization

T H Ellis  1 , D Lee, C M Thomas, P R Simpson, W G Cleary, M A Newman, K W Burcham
Affiliations

5S rRNA genes in Pisum: sequence, long range and chromosomal organization

T H Ellis et al. Mol Gen Genet. 1988 Oct.

Abstract

We have employed a combination of techniques to examine the organization of pea 5S rRNA genes. These include the analysis of length variant interspersion patterns in cosmid clones, sequence analysis, Southern analysis of both conventional gels and field inversion gels and in situ hybridization. From these analyses we conclude that the 5S rRNA genes of pea are arranged in three major tandem arrays which are represented by three large EcoRI fragments and that these correspond to the three sites of in situ hybridization in the haploid pea complement.

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