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. 2020 Nov;18(11):2167-2169.
doi: 10.1111/pbi.13395. Epub 2020 Jun 17.

Precision genome engineering in rice using prime editing system

Kai Hua  1 , Yuwei Jiang  1   2 , Xiaoping Tao  1 , Jian-Kang Zhu  1   3
Affiliations

Precision genome engineering in rice using prime editing system

Kai Hua et al. Plant Biotechnol J. 2020 Nov.
No abstract available

Keywords: Cas9; prime editing; prime editor; reverse transcriptase; rice.

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Conflict of interest statement

The authors declare no conflict of interests.

Figures

Figure 1
Figure 1
Developing a prime editing system for precision genome engineering in rice. (a) Schematic representation of different prime editors used in this study. (b) Diagram of the inactive GFP reporter. (c) The pegRNAs targeting the GFP inactive site designed for the different prime editors. (d) The ratios of GFP positive calli generated by different prime editors. (e) Detecting the GFP signal in transgenic rice calli under the fluorescence microscopy. In CK, the rice callus was not transformed by prime editors. Scale bars, 500 μm. (f) Sequence chromatograms of rice calli with or without GFP signals at GFP inactive site edited by Sp‐PE2. (g) Sequence chromatograms of rice calli with or without GFP signals at GFP inactive site edited by Sp‐PE3. (h) Sequence chromatogram of Line 14 at the GFP inactive site. This line was edited by Sp‐PE2. (i) The pegRNAs targeting ALS S627 site designed for prime editors Sp‐PE3 and Sa‐PE3. (j) Sequence chromatograms of Line 1 and Line 24 at ALS S627 site edited by Sp‐PE3. Line 1 was used as a control. (k) Sequence chromatograms of rice calli with or without GFP signals at GFP inactive site edited by Sa‐PE3. (l) Statistics of prime editing efficiencies at different target sites. PAM sequences recognized by SpCas9 or SaCas9 were marked by red or blue letters (c, i). Arrows point to the positions with an edited base (f, g, h, j, k).
See this image and copyright information in PMC

References

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