This site needs JavaScript to work properly. Please enable it to take advantage of the complete set of features!

Skip to main page content

Add to Collections

Your saved search

Name of saved search:

Search terms:

Test search terms

Would you like email updates of new search results?

Yes
No

Email: (change)

Frequency:

Which day?

Which day?

Report format:

Send at most:

Send even when there aren't any new results

Optional text in email:

Your RSS Feed

. 2020 Sep 15;65(17):1436-1439.

doi: 10.1016/j.scib.2020.04.041. Epub 2020 May 5.

Rapid and sensitive detection of COVID-19 using CRISPR/Cas12a-based detection with naked eye readout, CRISPR/Cas12a-NER

Xinjie Wang¹, Mingtian Zhong², Yong Liu³, Peixiang Ma¹, Lu Dang⁴, Qingzhou Meng⁴, Wenwei Wan⁵, Xiaodong Ma², Jia Liu¹, Guang Yang¹, Zifeng Yang⁶, Xingxu Huang⁷, Ming Liu⁸

Affiliations

Affiliations

¹ Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai 201210, China.
² Institute for Brain Research and Rehabilitation, Guangdong Key Laboratory of Mental Health and Cognitive Science, Center for Studies of Psychological Application, South China Normal University, Guangzhou 510631, China.
³ State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital, Guangzhou Medical University, Guangzhou 510120, China; Kingmed Virology Diagnostic & Translational Center, Guangzhou Kingmed Center for Clinical Laboratory Co., Ltd., Guangzhou 510005, China.
⁴ Affiliated Cancer Hospital & Institute of Guangzhou Medical University, Guangzhou 510095, China.
⁵ School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China.
⁶ State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital, Guangzhou Medical University, Guangzhou 510120, China. Electronic address: jeffyah@163.com.
⁷ School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China. Electronic address: huangxx@shanghaitech.edu.cn.
⁸ State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital, Guangzhou Medical University, Guangzhou 510120, China. Electronic address: mingliu128@hotmail.com.

PMID: 32373393
PMCID: PMC7198415
DOI: 10.1016/j.scib.2020.04.041

Rapid and sensitive detection of COVID-19 using CRISPR/Cas12a-based detection with naked eye readout, CRISPR/Cas12a-NER

Xinjie Wang et al. Sci Bull (Beijing). 2020.

. 2020 Sep 15;65(17):1436-1439.

doi: 10.1016/j.scib.2020.04.041. Epub 2020 May 5.

Authors

Xinjie Wang¹, Mingtian Zhong², Yong Liu³, Peixiang Ma¹, Lu Dang⁴, Qingzhou Meng⁴, Wenwei Wan⁵, Xiaodong Ma², Jia Liu¹, Guang Yang¹, Zifeng Yang⁶, Xingxu Huang⁷, Ming Liu⁸

Affiliations

¹ Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai 201210, China.
² Institute for Brain Research and Rehabilitation, Guangdong Key Laboratory of Mental Health and Cognitive Science, Center for Studies of Psychological Application, South China Normal University, Guangzhou 510631, China.
³ State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital, Guangzhou Medical University, Guangzhou 510120, China; Kingmed Virology Diagnostic & Translational Center, Guangzhou Kingmed Center for Clinical Laboratory Co., Ltd., Guangzhou 510005, China.
⁴ Affiliated Cancer Hospital & Institute of Guangzhou Medical University, Guangzhou 510095, China.
⁵ School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China.
⁶ State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital, Guangzhou Medical University, Guangzhou 510120, China. Electronic address: jeffyah@163.com.
⁷ School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China. Electronic address: huangxx@shanghaitech.edu.cn.
⁸ State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital, Guangzhou Medical University, Guangzhou 510120, China. Electronic address: mingliu128@hotmail.com.

PMID: 32373393
PMCID: PMC7198415
DOI: 10.1016/j.scib.2020.04.041

No abstract available

PubMed Disclaimer

Conflict of interest statement

The author declares that they have no conflict of interest.

Figures

Fig. 1 — Fig. 1
CRISPR/Cas12a-based detection with naked eye readout (CRISPR/Cas12a-NER) of SARS-CoV-2. (a) Schematic of Cas12a-based assay for rapid and visual nucleic acid detection. After 30 min of RT-RAA reaction, the Cas12a enzyme targets cleavage for an additional 15 min. The green fluorescent signals can be observed by the naked eye under blue light with a wavelength of 485 nm. (b) Four target gene fragments and SARS-CoV-2-specific crRNAs location on the genome. (c) Validation of crRNA specifically reacts with the target gene. (d) Time course of E gene detection with E-crRNAmix on gradient diluted input RNAs from 1e4 to 1e0 copies. (e) Sensitivity assay using E-crRNAmix to detect gradient diluted E gene RNA from 1e4 to 1e0 copies. (f) Diagnostic accuracy of CRISPR/Cas12a-NER on the diagnosis of COVID-19 clinical samples. ***: statistical significance; NS: not significance.

See this image and copyright information in PMC

References

1. Lu R., Zhao X., Li J. Genomic characterisation and epidemiology of 2019 novel coronavirus: implications for virus origins and receptor binding. Lancet. 2020;395:565–574. - PMC - PubMed
1. Wu F., Zhao S., Yu B. A new coronavirus associated with human respiratory disease in China. Nature. 2020;579:265–269. - PMC - PubMed
1. Chu D.K.W., Pan Y., Cheng S.M.S. Molecular diagnosis of a novel coronavirus (2019-ncov) causing an outbreak of pneumonia. Clin Chem. 2020;66:549–555. - PMC - PubMed
1. Jin Y.H., Cai L., Cheng Z.S. A rapid advice guideline for the diagnosis and treatment of 2019 novel coronavirus (2019-ncov) infected pneumonia (standard version) Mil Med Res. 2020;7:4. - PMC - PubMed
1. Gootenberg J.S., Abudayyeh O.O., Lee J.W. Nucleic acid detection with crispr-cas13a/c2c2. Science. 2017;356:438–442. - PMC - PubMed

LinkOut - more resources

Full Text Sources
Other Literature Sources
- The Lens - Patent Citations Database