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. 2020 Sep 15;65(17):1436-1439.

doi: 10.1016/j.scib.2020.04.041. Epub 2020 May 5.

Rapid and sensitive detection of COVID-19 using CRISPR/Cas12a-based detection with naked eye readout, CRISPR/Cas12a-NER

Xinjie Wang¹, Mingtian Zhong², Yong Liu³, Peixiang Ma¹, Lu Dang⁴, Qingzhou Meng⁴, Wenwei Wan⁵, Xiaodong Ma², Jia Liu¹, Guang Yang¹, Zifeng Yang⁶, Xingxu Huang⁷, Ming Liu⁸

Affiliations

Affiliations

¹ Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai 201210, China.
² Institute for Brain Research and Rehabilitation, Guangdong Key Laboratory of Mental Health and Cognitive Science, Center for Studies of Psychological Application, South China Normal University, Guangzhou 510631, China.
³ State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital, Guangzhou Medical University, Guangzhou 510120, China; Kingmed Virology Diagnostic & Translational Center, Guangzhou Kingmed Center for Clinical Laboratory Co., Ltd., Guangzhou 510005, China.
⁴ Affiliated Cancer Hospital & Institute of Guangzhou Medical University, Guangzhou 510095, China.
⁵ School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China.
⁶ State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital, Guangzhou Medical University, Guangzhou 510120, China. Electronic address: jeffyah@163.com.
⁷ School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China. Electronic address: huangxx@shanghaitech.edu.cn.
⁸ State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital, Guangzhou Medical University, Guangzhou 510120, China. Electronic address: mingliu128@hotmail.com.

PMID: 32373393
PMCID: PMC7198415
DOI: 10.1016/j.scib.2020.04.041

Rapid and sensitive detection of COVID-19 using CRISPR/Cas12a-based detection with naked eye readout, CRISPR/Cas12a-NER

Xinjie Wang et al. Sci Bull (Beijing). 2020.

. 2020 Sep 15;65(17):1436-1439.

doi: 10.1016/j.scib.2020.04.041. Epub 2020 May 5.

Authors

Xinjie Wang¹, Mingtian Zhong², Yong Liu³, Peixiang Ma¹, Lu Dang⁴, Qingzhou Meng⁴, Wenwei Wan⁵, Xiaodong Ma², Jia Liu¹, Guang Yang¹, Zifeng Yang⁶, Xingxu Huang⁷, Ming Liu⁸

Affiliations

¹ Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai 201210, China.
² Institute for Brain Research and Rehabilitation, Guangdong Key Laboratory of Mental Health and Cognitive Science, Center for Studies of Psychological Application, South China Normal University, Guangzhou 510631, China.
³ State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital, Guangzhou Medical University, Guangzhou 510120, China; Kingmed Virology Diagnostic & Translational Center, Guangzhou Kingmed Center for Clinical Laboratory Co., Ltd., Guangzhou 510005, China.
⁴ Affiliated Cancer Hospital & Institute of Guangzhou Medical University, Guangzhou 510095, China.
⁵ School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China.
⁶ State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital, Guangzhou Medical University, Guangzhou 510120, China. Electronic address: jeffyah@163.com.
⁷ School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China. Electronic address: huangxx@shanghaitech.edu.cn.
⁸ State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital, Guangzhou Medical University, Guangzhou 510120, China. Electronic address: mingliu128@hotmail.com.

PMID: 32373393
PMCID: PMC7198415
DOI: 10.1016/j.scib.2020.04.041

No abstract available

PubMed Disclaimer

Conflict of interest statement

The author declares that they have no conflict of interest.

Figures

Fig. 1 — Fig. 1
CRISPR/Cas12a-based detection with naked eye readout (CRISPR/Cas12a-NER) of SARS-CoV-2. (a) Schematic of Cas12a-based assay for rapid and visual nucleic acid detection. After 30 min of RT-RAA reaction, the Cas12a enzyme targets cleavage for an additional 15 min. The green fluorescent signals can be observed by the naked eye under blue light with a wavelength of 485 nm. (b) Four target gene fragments and SARS-CoV-2-specific crRNAs location on the genome. (c) Validation of crRNA specifically reacts with the target gene. (d) Time course of E gene detection with E-crRNAmix on gradient diluted input RNAs from 1e4 to 1e0 copies. (e) Sensitivity assay using E-crRNAmix to detect gradient diluted E gene RNA from 1e4 to 1e0 copies. (f) Diagnostic accuracy of CRISPR/Cas12a-NER on the diagnosis of COVID-19 clinical samples. ***: statistical significance; NS: not significance.

See this image and copyright information in PMC

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