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. 2020 Jun;10(2):297-306.
doi: 10.34172/apb.2020.036. Epub 2020 Feb 18.

Effect of Hypoxia Preconditioned Adipose-Derived Mesenchymal Stem Cell Conditioned Medium on Cerulein-Induced Acute Pancreatitis in Mice

Kamal Abdolmohammadi  1 , Tayebeh Mahmoudi  2 , Shahrzad Nojehdehi  3 , Lobat Tayebi  4 , Seyed Mahmoud Hashemi  5   6   7 , Farshid Noorbakhsh  1 , Alireza Abdollahi  8   9 , Masoud Soleimani  10 , Behrouz Nikbin  1   11 , Mohammad Hossein Nicknam  1   11
Affiliations

Effect of Hypoxia Preconditioned Adipose-Derived Mesenchymal Stem Cell Conditioned Medium on Cerulein-Induced Acute Pancreatitis in Mice

Kamal Abdolmohammadi et al. Adv Pharm Bull. 2020 Jun.

Abstract

Purpose: Acute pancreatitis (AP) is an inflammatory disorder distinguished by tissue injury and inflammation of the pancreas. Using paracrine potential of mesenchymal stem cells (MSCs) provides a useful clinical approach in treating inflammatory diseases. We investigated the therapeutic effects of adipose-derived MSC conditioned medium (CM) and hypoxia preconditioned adipose-derived MSC conditioned medium (HCM) in cerulein-induced AP in mice. Methods: AP was induced in C57BL/6 mice by intraperitoneal injection of cerulein (75 μg/ kg/h × 7 times). One hour following the last injection of cerulein, mice were treated with intraperitoneal injection of CM and HCM (500 µL/mice/30 min × 3 times). Twelve hours following the treatment, serum levels of amylase and lipase were measured. In addition, pancreas pathological changes, immunohistochemical examinations for evaluation of IL-6 expression and pancreatic myeloperoxidase (MPO) enzyme activity were analyzed. Results: The in vitro results of the morphological, differentiation and immunophenotyping analyses confirmed that hypoxia preconditioned MSCs (HP-MSCs) conserve MSCs characteristics after preconditioning. However, HP-MSCs significantly expressed high mRNA level of hypoxia inducible factor 1-α and higher level of total protein. The in vivo findings of the current study showed that CM and HCM significantly reduced the amylase & lipase activity, the severity of pancreas tissue injury and the expression of IL-6 and MPO enzyme activity compared with the AP group. However, no significant difference between CM and HCM groups was demonstrated. Conclusion: Use of CM and HCM can attenuate cerulein-induced AP and decrease inflammation in the pancreas tissue in AP mice.

Keywords: Acute Pancreatitis; Cerulein; Conditioned Medium; Inflammation; Mesenchymal Stem Cell; Preconditioning.

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References

    1. Márta K, Lazarescu AM, Farkas N, Mátrai P, Cazacu I, Ottóffy M. et al. Aging and comorbidities in acute pancreatitis I: a meta-analysis and systematic review based on 194,702 patients. Front Physiol. 2019;10:328. doi: 10.3389/fphys.2019.00328. - DOI - PMC - PubMed
    1. Tenner S, Baillie J, DeWitt J, Vege SS. American College of Gastroenterology guideline: management of acute pancreatitis. Am J Gastroenterol. 2013;108(9):1400–15; 16. doi: 10.1038/ajg.2013.218. - DOI - PubMed
    1. Pasari LP, Khurana A, Anchi P, Aslam Saifi M, Annaldas S, Godugu C. Visnagin attenuates acute pancreatitis via Nrf2/NFkappaB pathway and abrogates associated multiple organ dysfunction. Biomed Pharmacother. 2019;112:108629. doi: 10.1016/j.biopha.2019.108629. - DOI - PubMed
    1. Bhatia M, Wong FL, Cao Y, Lau HY, Huang J, Puneet P. et al. Pathophysiology of acute pancreatitis. Pancreatology. 2005;5(2-3):132–44. doi: 10.1159/000085265. - DOI - PubMed
    1. Sah RP, Saluja A. Molecular mechanisms of pancreatic injury. Curr Opin Gastroenterol. 2011;27(5):444–51. doi: 10.1097/MOG.0b013e328349e346. - DOI - PMC - PubMed

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