Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2020 Apr;24(8):4320-4327.
doi: 10.26355/eurrev_202004_21013.

Circ_0067934 correlates with poor prognosis and promotes laryngeal squamous cell cancer progression by sponging miR-1324

Y-L Chu  1
Affiliations
Free article

Circ_0067934 correlates with poor prognosis and promotes laryngeal squamous cell cancer progression by sponging miR-1324

Y-L Chu. Eur Rev Med Pharmacol Sci. 2020 Apr.
Free article

Abstract

Objective: As a kind of non-coding RNA, circular RNA (circRNA) plays a regulatory role in many tumors. Our intention was to investigate the clinical significance, biological function, and molecular regulation mechanisms of circ_0067934 in laryngeal squamous cell cancer (LSCC) progression.

Patients and methods: The expression levels of circ_0067934 in LSCC tumor samples and cells were determined by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). The associations of circ_0067934 expression with clinicopathological features and overall survival in LSCC patients were statistically analyzed. The biological function of circ_0067934 in LSCC cells was analyzed by CCK-8, colony formation, EdU, and transwell assays. Dual-Luciferase reporter assay was conducted to verify that circ_0067934 may sponge miR-1324 to regulate proliferation and migration of LSCC.

Results: Circ_0067934 expression was remarkably upregulated in LSCC tissues and cells. High level of circ_0067934 was significantly related to tumor size, lymph node status, and distant metastasis of LSCC. Furthermore, highly expressed circ_0067934 could result in notably worse survival. In vitro experiments showed that down-regulation of circ_0067934 inhibited proliferation and migration of LSCC cells. Mechanism analysis revealed that circ_0067934 regulated LSCC development by sponging miR-1324.

Conclusions: Circ_0067934 may function as an oncogene in LSCC, which provided a feasible prognostic biomarker and therapeutic target for LSCC.

PubMed Disclaimer