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. 2002 Jun;12(2):187-192.
doi: 10.1023/A:1016800515030.

On the Possibility of Glucose Sensing Using Boronic Acid and a Luminescent Ruthenium Metal-Ligand Complex

Zakir Murtaza  1 , Leah Tolosa  1 , Peter Harms  1 , Joseph R Lakowicz  1
Affiliations

On the Possibility of Glucose Sensing Using Boronic Acid and a Luminescent Ruthenium Metal-Ligand Complex

Zakir Murtaza et al. J Fluoresc. 2002 Jun.

Abstract

We describe a new approach to optical sensing of glucose based on the competitive interactions between a ruthenium metal ligand complex, a boronic acid derivative and glucose. The metal-ligand complex [Ru(2,2'-bipyridme)2(5,6-dihydroxy-1,10-phenanthrolme)](PF6)2 at pH 8 forms a reversible complex with 2-toluylboronic acid or 2-methoxyphenyl boronic acid. Complexation is accompanied by a several-fold increase in the luminescent intensity of the ruthenium complex. Addition of glucose results in decreased luminescent intensity, which appears to be the result of decreased binding between the metal-ligand complex and the boronic acid. Ruthenium metal-ligand complexes are convenient for optical sensing because their long luminescent decay times allow lifetime-based sensing with simple instrumentation.

Keywords: Glucose sensing; boronic acid; metal-ligand complex.

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Figures

Fig. 1.
Fig. 1.
Emission spectra of 45μM [Ru(bpy)2dhph]2+ in the presence of increasing concentrations of TBA at pH 8.0.
Fig. 2.
Fig. 2.
Effect of increasing concentrations of glucose on the total intensity of 45 μM [Ru(bpy)2dhph]2+ in the presence of 45 μM TBA at pH 8.0.
Fig. 3.
Fig. 3.
Emission spectra of 45 μM [Ru(bpy)2dhph]2+ in the presence of increasing concentrations of MBA at pH 8.0.
Fig. 4.
Fig. 4.
Normalized total intensities of 45 μM [Ru(bpy)2dhph]2+ calculated from the spectra in Figs. 1 and 3, showing the relative reversibilities of the reaction of the TBA and MBA to [Ru(bpy)2dhph]2+.
Fig. 5.
Fig. 5.
Emission spectra of 45 μM [Ru(bpy)2dhph]2+ + 180 μM MBA with increasing glucose concentrations at pH 8.0. Insert: Intensities as a function of glucose concentration.
Fig. 6.
Fig. 6.
Frequency-domain intensity decays 45 μM [Ru(bpy)2dhph]2+ in the presence of increasing concentrations of MBA at pH 8.0.
Scheme 1.
Scheme 1.
Analytical scheme for sensing glucose based on competitive binding with [Ru(bpy)2dhph]2+ and boronic acid.
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