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. 2020 May;61(3):297-303.
doi: 10.4111/icu.2020.61.3.297. Epub 2020 Mar 25.

Expression of bladder α1-adrenoceptor subtype after relief of partial bladder outlet obstruction in a rat model

Ji Yong Lee  1 , Jong Mok Park  1 , Yong Gil Na  1 , Ki Hak Song  1 , Jae Sung Lim  1 , Seung Woo Yang  1 , Gun Hwa Kim  2   3 , Ju Hyun Shin  1
Affiliations

Expression of bladder α1-adrenoceptor subtype after relief of partial bladder outlet obstruction in a rat model

Ji Yong Lee et al. Investig Clin Urol. 2020 May.

Abstract

Purpose: Many patients with benign prostatic hyperplasia require treatment for persistent storage symptoms, even when the obstruction is successfully relieved by surgery. Previous studies identified a characteristic increase in α1D-adrenoceptor levels in the bladder in a bladder outlet obstruction (BOO) model. Here, we investigated the expression of α1-adrenoceptor subtypes in the bladder after relief of partial BOO (pBOO) in a rat model.

Materials and methods: A total of 60 female Sprague-Dawley rats were randomly divided into three groups (sham-operated, pBOO, and pBOO relief groups), and the expression of α1-adrenoceptor subtypes in the urothelium and detrusor muscle tissues was examined by western blot.

Results: The expression of the α1D-adrenoceptor was significantly higher in the urothelium and detrusor muscle tissue of the pBOO and pBOO relief groups than in the corresponding tissue of the sham-operated group. Additionally, the α1A-adrenoceptor was predominant in the sham-operated group but significantly decreased in the urothelium in the pBOO group. No significant differences were found in α1A-adrenoceptor levels in detrusor muscle or whole bladder.

Conclusions: Our results showed that α1D-adrenoceptor levels were consistently increased with pBOO, even after relief, suggesting that the α1D-adrenoceptor might be a cause of persistent storage symptoms after relief of pBOO.

Keywords: Adrenergic alpha-1 receptor antagonists; Lower urinary tract symptoms; Urinary bladder neck obstruction; Urinary bladder, overactive.

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Conflict of interest statement

CONFLICTS OF INTEREST: The authors have nothing to disclose.

Figures

Fig. 1
Fig. 1. Western blot analysis of α1-adrenoceptor (AR) subtypes in the urothelium. (A) Immunoblot of α1-AR subtypes and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) in representative animals. (B) Bar graph showing data for the urothelium from all animals in each group. In the pBOO and the pBOO+R groups, α1A-AR levels were significantly decreased, and α1D-AR levels were significantly increased, relative to the Sham group. α1D-AR levels were significantly decreased in the pBOO+R group relative to the pBOO group. Data represent the mean±standard deviation. *p<0.05 vs. Sham group; †p<0.05 vs. pBOO group. BOO, bladder outlet obstruction; pBOO group, rat model of partial BOO; pBOO+R group, rat model of partial BOO relief.
Fig. 2
Fig. 2. Western blot analysis of α1-adrenoceptor (AR) subtypes in the detrusor muscle. (A) Immunoblot of α1-AR subtypes and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) in representative animals. (B) Bar graph showing data for the detrusor muscle from all animals in each group. In the pBOO and pBOO+R groups, α1D-AR levels were significantly increased relative to the Sham group, and differences in α1A- and α1B-AR levels were not statistically significant among the three groups. Data represent the mean±standard deviation. *p<0.05 vs. Sham group. BOO, bladder outlet obstruction; pBOO group, rat model of partial BOO; pBOO+R group, rat model of partial BOO relief.
Fig. 3
Fig. 3. Average relative density of α1-adrenoceptor (AR) protein levels in the whole bladder. The bar graph shows data for the urothelium and detrusor muscle tissue represented as the mean±standard deviation. In the pBOO and pBOO+R groups, α1D-AR levels were significantly increased relative to the Sham group and significantly decreased in the pBOO+R group relative to the pBOO group. *p<0.05 vs. Sham group; †p<0.05 vs. pBOO group. BOO, bladder outlet obstruction; pBOO group, rat model of partial BOO; pBOO+R group, rat model of partial BOO relief.
See this image and copyright information in PMC

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