doi: 10.1128/MRA.00156-20.
Complete Genome Sequence of Tetracycline-Resistant Serratia liquefaciens S1, Isolated from Mixed Greens, Obtained Using Illumina MiSeq and Oxford Nanopore MinION Sequencing
Affiliations
- PMID: 32381605
- PMCID: PMC7206483
- DOI: 10.1128/MRA.00156-20
Item in Clipboard
Complete Genome Sequence of Tetracycline-Resistant Serratia liquefaciens S1, Isolated from Mixed Greens, Obtained Using Illumina MiSeq and Oxford Nanopore MinION Sequencing
Microbiol Resour Announc.
.
Abstract
Serratia liquefaciens strain S1 was isolated from mixed greens and sequenced with short-read Illumina MiSeq technology and long-read MinION technology. Hybrid sequence assembly generated a complete single circular chromosome and two extrachromosomal contigs, which together encoded 5,098 proteins, 85 tRNAs, and 22 rRNAs.
Copyright © 2020 Stein et al.
Similar articles
-
Evaluation of strategies for the assembly of diverse bacterial genomes using MinION long-read sequencing.BMC Genomics. 2019 Jan 9;20(1):23. doi: 10.1186/s12864-018-5381-7. BMC Genomics. 2019. PMID: 30626323 Free PMC article.
-
Resolving plasmid structures in Enterobacteriaceae using the MinION nanopore sequencer: assessment of MinION and MinION/Illumina hybrid data assembly approaches.Microb Genom. 2017 Jun 9;3(8):e000118. doi: 10.1099/mgen.0.000118. eCollection 2017 Aug. Microb Genom. 2017. PMID: 29026658 Free PMC article.
-
Genome assembly using Nanopore-guided long and error-free DNA reads.BMC Genomics. 2015 Apr 20;16(1):327. doi: 10.1186/s12864-015-1519-z. BMC Genomics. 2015. PMID: 25927464 Free PMC article.
-
High precision genome sequencing of engineered Gluconobacter oxydans 621H by combining long nanopore and short accurate Illumina reads.J Biotechnol. 2017 Sep 20;258:197-205. doi: 10.1016/j.jbiotec.2017.04.016. Epub 2017 Apr 19. J Biotechnol. 2017. PMID: 28433722
-
Fusobacterium Genomics Using MinION and Illumina Sequencing Enables Genome Completion and Correction.mSphere. 2018 Jul 5;3(4):e00269-18. doi: 10.1128/mSphere.00269-18. mSphere. 2018. PMID: 29976645 Free PMC article.
Cited by
-
Complete Genome Sequence of a Shiga Toxin-Producing Escherichia coli O26:H11 Strain (Sequence Type 21) and Two Draft Genome Sequences of Listeria monocytogenes Strains (Clonal Complex 1 [CC1] and CC59) Isolated from Fresh Produce in Germany.Microbiol Resour Announc. 2020 Dec 3;9(49):e00973-20. doi: 10.1128/MRA.00973-20. Microbiol Resour Announc. 2020. PMID: 33272986 Free PMC article.
References
-
- Ashelford KE, Fry JC, Bailey MJ, Day MJ. 2002. Characterization of Serratia isolates from soil, ecological implications and transfer of Serratia proteamaculans subsp. quinovora Grimont et al. 1983 to Serratia quinivorans corrig., sp. nov. Int J Syst Evol Microbiol 52:2281–2289. doi:10.1099/00207713-52-6-2281. - DOI - PubMed
-
- Walker BJ, Abeel T, Shea T, Priest M, Abouelliel A, Sakthikumar S, Cuomo CA, Zeng Q, Wortman J, Young SK, Earl AM. 2014. Pilon: an integrated tool for comprehensive microbial variant detection and genome assembly improvement. PLoS One 9:e112963. doi:10.1371/journal.pone.0112963. - DOI - PMC - PubMed
LinkOut - more resources
Full Text Sources
Molecular Biology Databases
